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Literature summary for 3.4.24.30 extracted from

  • Fernandez-Espla, M.D.; Garault, P.; Monnet, V.; Rul, F.
    Streptococcus thermophilus cell wall-anchored proteinase: release, purification, and biochemical and genetic characterization (2000), Appl. Environ. Microbiol., 66, 4772-4778.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.30

Application

Application Comment Organism
nutrition wide utilization in the production of dairy products like yoghurt, hard cooked cheese, and soft cheese Streptococcus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
prtS gene sequenced by performing PCR Streptococcus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
chymostatin 75% inhibition Streptococcus thermophilus
EDTA 30% inhibition Streptococcus thermophilus
iodoacetic acid 83% inhibition Streptococcus thermophilus
additional information no inhibition with bestatin or E64 Streptococcus thermophilus
phenylmethylsulfonyl fluoride strongly inhibited, 100% inhibition Streptococcus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ highly activated by Ca2+ ions Streptococcus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
153000
-
 purified enzyme Streptococcus thermophilus
169000
-
 deduced from nucleotide sequence of prtS Streptococcus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-casein + H2O Streptococcus thermophilus
-
 ?
-
 ?
beta-casein + H2O Streptococcus thermophilus CNRZ 385
-
 ?
-
 ?
casein + H2O Streptococcus thermophilus
-
 ?
-
 ?
casein + H2O Streptococcus thermophilus CNRZ 385
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus thermophilus Q9F8Q4 CNRZ 385
-
Streptococcus thermophilus CNRZ 385 Q9F8Q4 CNRZ 385
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methoxysuccinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
-
 Streptococcus thermophilus ?
-
 ?
3-methoxysuccinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
-
 Streptococcus thermophilus CNRZ 385 ?
-
 ?
acetyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Streptococcus thermophilus ?
-
 ?
acetyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
-
 Streptococcus thermophilus CNRZ 385 ?
-
 ?
beta-casein + H2O
-
 Streptococcus thermophilus ?
-
 ?
beta-casein + H2O
-
 Streptococcus thermophilus CNRZ 385 ?
-
 ?
casein + H2O
-
 Streptococcus thermophilus ?
-
 ?
casein + H2O
-
 Streptococcus thermophilus CNRZ 385 ?
-
 ?
additional information Bz-Phe-Val-Arg-p-nitroanilide, NZbz-Gly-Pro-Arg-p-nitroanilide, and Z-Phe-Arg-p-nitroanilide are no substrates Streptococcus thermophilus ?
-
 ?
additional information Bz-Phe-Val-Arg-p-nitroanilide, NZbz-Gly-Pro-Arg-p-nitroanilide, and Z-Phe-Arg-p-nitroanilide are no substrates Streptococcus thermophilus CNRZ 385 ?
-
 ?
succinyl-Ala-Glu-Pro-Phe-p-nitroanilide + H2O
-
 Streptococcus thermophilus ?
-
 ?
succinyl-Val-Pro-Phe-p-nitroanilide + H2O
-
 Streptococcus thermophilus ?
-
 ?

Synonyms

Synonyms Comment Organism
cell envelope protein
-
 Streptococcus thermophilus
cell wall-anchored proteinase
-
 Streptococcus thermophilus
CEP
-
 Streptococcus thermophilus
PrtS
-
 Streptococcus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Streptococcus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Streptococcus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 about 70% activity remaining at pH 7.0 and pH 8.0 Streptococcus thermophilus