Protein Variants | Comment | Organism |
---|---|---|
G8C/N60C | double mutant temperature optimum at 80°C, increased thermal stability | Geobacillus stearothermophilus |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 48 h, 37°C, 50% residual activity with 44.5% (v/v) and 65.5% (v/v) for pseudo wild-type and mutant G8C/N60C respectively | Geobacillus stearothermophilus |
Acetone | 48 h, 37°C, 50% residual activity with 30.0% (v/v) and 50.1% (v/v) for pseudo wild-type and mutant G8C/N60C respectively | Geobacillus stearothermophilus |
acetonitrile | 48 h, 37°C, 50% residual activity with 30.6% (v/v) and 51.6% (v/v) for pseudo wild-type and mutant G8C/N60C respectively | Geobacillus stearothermophilus |
dimethylformamide | 48 h, 37°C, 50% residual activity with 29.8% (v/v) and 45.8% (v/v) for pseudo wild-type and mutant G8C/N60C respectively | Geobacillus stearothermophilus |
dioxane | 48 h, 37°C, 50% residual activity with 32.1% (v/v) and 47.9% (v/v) for pseudo wild-type and mutant G8C/N60C respectively | Geobacillus stearothermophilus |
DMSO | 48 h, 37°C, 50% residual activity with 32.9% (v/v) and 48.7% (v/v) for pseudo wild-type and mutant G8C/N60C respectively | Geobacillus stearothermophilus |
Methanol | 48 h, 37°C, 50% residual activity with 56.6% (v/v) and 86.4% (v/v) for pseudo wild-type and mutant G8C/N60C respectively | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
78.5 | - |
Boilysin at 37°C with casein as substrate | Geobacillus stearothermophilus |
81.5 | - |
pseudo wild type at 37°C with casein as substrate | Geobacillus stearothermophilus |
81.9 | - |
wild type enzyme at 37°C with casein as substrate | Geobacillus stearothermophilus |
82.4 | - |
double mutant G8C/N60C at 37°C with casein as substrate | Geobacillus stearothermophilus |
242 | - |
wild type enzyme at temperature optimum 75°C | Geobacillus stearothermophilus |
273 | - |
pseudo wild type at temperature optimum 75°C | Geobacillus stearothermophilus |
375 | - |
double mutant G8C/N60C at temperature optimum 80°C | Geobacillus stearothermophilus |
608 | - |
boilysin at temperature optimum 95°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(2-furylacryloyl)-glycyl-L-leucinamide + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
casein + H2O | - |
Geobacillus stearothermophilus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
wild type enzyme | Geobacillus stearothermophilus |
75 | - |
pseudo wild type | Geobacillus stearothermophilus |
80 | - |
double mutant G8C/N60C | Geobacillus stearothermophilus |
95 | - |
boilysin | Geobacillus stearothermophilus |