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Literature summary for 3.4.24.28 extracted from

  • Van den Burg, B.; Eijsink, V.G.H.; Vriend, G.; Veltman, O.R.; Venema, G.
    Rendering one autolysis site in Bacillus subtilis neutral protease resistant to cleavage reveals a new fission (1998), Biotechnol. Appl. Biochem., 27, 125-132.
No PubMed abstract available
Search for more literature for 3.4.24.28

Cloned(Commentary)

Cloned (Comment) Organism
protease-deficient strain Bacillus subtilis DB117 used as host for plasmids, subcloned in Escherichia coli for site-directed mutagenesis Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
I157D site-directed mutagenesis Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
N-[alpha-L-rhamnopyranosyl-oxyhydroxyphosphinyl]-L-leucyl-L-tryptophan
-
 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide pH 7.5, 37°C, mutant I157D Bacillus subtilis
0.3
-
 3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide pH 7.5, 37°C, wild-type Bacillus subtilis
1.3
-
 3-(2-furylacryloyl)-glycyl-L-leucine amide pH 7.5, 37°C, wild-type Bacillus subtilis
1.4
-
 3-(2-furylacryloyl)-glycyl-L-leucine amide pH 7.5, 37°C, mutant I157D Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant TLPs Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(2-furylacryloyl)-glycyl-L-leucine amide + H2O
-
 Bacillus subtilis ?
-
 ?
3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide + H2O
-
 Bacillus subtilis ?
-
 ?
beta-casein + H2O
-
 Bacillus subtilis hydrolyzed casein
-
 ?
insulin B chain + H2O
-
 Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
thermoysin-like proteinase
-
 Bacillus subtilis
TLP-sub
-
 Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58.6
-
 T50 value at pH 7.0 Bacillus subtilis
59.5
-
 T50 value at pH 5.3 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
64
-
 3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide pH 7.5, 37°C, wild-type Bacillus subtilis
80
-
 3-(2-furylacryloyl)-glycyl-L-leucine amide pH 7.5, 37°C, mutant I157D Bacillus subtilis
99
-
 3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide pH 7.5, 37°C, mutant I157D Bacillus subtilis
256
-
 3-(2-furylacryloyl)-glycyl-L-leucine amide pH 7.5, 37°C, wild-type Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0035
-
 N-[alpha-L-rhamnopyranosyl-oxyhydroxyphosphinyl]-L-leucyl-L-tryptophan pH 7.5, 37°C, wild-type Bacillus subtilis
0.0037
-
 N-[alpha-L-rhamnopyranosyl-oxyhydroxyphosphinyl]-L-leucyl-L-tryptophan pH 7.5, 37°C, mutant I157D Bacillus subtilis