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Literature summary for 3.4.24.27 extracted from

  • Osago, H.; Kobayashi-Miura, M.; Hamasaki, Y.; Hara, N.; Hiyoshi, M.; Tsuchiya, M.
    Complete solubilization of cartilage using the heat-stable protease thermolysin for comprehensive GAG analysis (2018), Anal. Biochem., 548, 115-118 .
    View publication on PubMed

Application

Application Comment Organism
analysis thermolysin offers a tool for complete solubilization of cartilage prior to comprehensive glycosaminoglycans(GAG)omic analysis, and is likely applicable to other collagen-rich tissues such as ligaments, skin, and blood vessels Bacillus thermoproteolyticus

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen + H2O collagen in solid articular cartilage at 70°C. Overnight digestion with thermolysin completely solubilized cartilage. Following thermolysin treatments, almost all glycosaminoglycans are extracted from the cartilage Bacillus thermoproteolyticus ?
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