Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dimethylformamide | - |
Bacillus thermoproteolyticus | |
DMSO | - |
Bacillus thermoproteolyticus | |
Isopropanol | - |
Bacillus thermoproteolyticus | |
n-Propanol | - |
Bacillus thermoproteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bacillus thermoproteolyticus | |
Zn2+ | - |
Bacillus thermoproteolyticus |
Organic Solvent | Comment | Organism |
---|---|---|
dimethylformamide | half-life is 6 min at 80°C, trehalose stabilizes the enzyme in DMF | Bacillus thermoproteolyticus |
DMSO | half-life is over 20 min at 80°C | Bacillus thermoproteolyticus |
isopropanol | half-life is 4 min at 80°C, stabilizes the enzyme | Bacillus thermoproteolyticus |
additional information | solvents cause mixed inhibition of thermolysin, kinetic and structural studies, overview | Bacillus thermoproteolyticus |
n-propanol | half-life is 3 min at 80°C, glycerol stabilizes the enzyme | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus thermoproteolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
thermoinactivation of thermolysin at 80°C in 50% of solvents, half-lives of 3-20 min, with the increase in solvent hydrophobicity, thermal stability of the enzyme decreases, overview | Bacillus thermoproteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bacillus thermoproteolyticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | organic solvent inhibition kinetics, overview | Bacillus thermoproteolyticus | |
0.18 | - |
n-Propanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
0.25 | - |
Isopropanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
7.63 | - |
dimethylformamide | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
7.7 | - |
DMSO | pH 7.5, 25°C | Bacillus thermoproteolyticus |