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Literature summary for 3.4.24.27 extracted from

  • Matsumiya, Y.; Nishikawa, K.; Aoshima, H.; Inouye, K.; Kubo, M.
    Analysis of autodegradation sites of thermolysin and enhancement of its thermostability by modifying Leu155 at an autodegradation site (2004), J. Biochem., 135, 547-553.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
L155A thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to A155-I156 and the bond I164-D165 is newly recognized as an autodegradation site Bacillus thermoproteolyticus
L155F thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to F155-I156 and the bond I164-D165 is newly recognized as an autodegradation site Bacillus thermoproteolyticus
L155G thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to G155-I156 and the bond I164-D165 is newly recognized as an autodegradation site Bacillus thermoproteolyticus
L155S thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to S155-I156 and the bond I164-D165 is newly recognized as an autodegradation site Bacillus thermoproteolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155G, 37°C, pH 7.0 Bacillus thermoproteolyticus
13
-
N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155S, 37°C, pH 7.0 Bacillus thermoproteolyticus
14
-
N-carbobenzoxy-Gly-L-Leu-NH2 wild-type, 37°C, pH 7.0 Bacillus thermoproteolyticus
17
-
N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155F, 37°C, pH 7.0 Bacillus thermoproteolyticus
20
-
N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155A, 37°C, pH 7.0 Bacillus thermoproteolyticus

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbobenzoxy-Gly-L-Leu-NH2 + H2O
-
Bacillus thermoproteolyticus ?
-
?

Subunits

Subunits Comment Organism
More in presence of Ca2+, enzyme exhibits four autodegradation sites, one of them being Gly154-Leu155 Bacillus thermoproteolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.3
-
N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155F, 37°C, pH 7.0 Bacillus thermoproteolyticus
51.6
-
N-carbobenzoxy-Gly-L-Leu-NH2 wild-type, 37°C, pH 7.0 Bacillus thermoproteolyticus
80
-
N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155A, 37°C, pH 7.0 Bacillus thermoproteolyticus
150
-
N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155G, 37°C, pH 7.0 Bacillus thermoproteolyticus
165
-
N-carbobenzoxy-Gly-L-Leu-NH2 mutant L155S, 37°C, pH 7.0 Bacillus thermoproteolyticus