Protein Variants | Comment | Organism |
---|---|---|
L155A | thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to A155-I156 and the bond I164-D165 is newly recognized as an autodegradation site | Bacillus thermoproteolyticus |
L155F | thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to F155-I156 and the bond I164-D165 is newly recognized as an autodegradation site | Bacillus thermoproteolyticus |
L155G | thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to G155-I156 and the bond I164-D165 is newly recognized as an autodegradation site | Bacillus thermoproteolyticus |
L155S | thermostability at 80°C increases with amino acid substitutions at L155 in the order of wild-type, Gly, Ser, Phe, Ala. Autodegradation site shifts from G154-L155 to S155-I156 and the bond I164-D165 is newly recognized as an autodegradation site | Bacillus thermoproteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155G, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
13 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155S, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
14 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | wild-type, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
17 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155F, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
20 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155A, 37°C, pH 7.0 | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbobenzoxy-Gly-L-Leu-NH2 + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | in presence of Ca2+, enzyme exhibits four autodegradation sites, one of them being Gly154-Leu155 | Bacillus thermoproteolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.3 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155F, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
51.6 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | wild-type, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
80 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155A, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
150 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155G, 37°C, pH 7.0 | Bacillus thermoproteolyticus | |
165 | - |
N-carbobenzoxy-Gly-L-Leu-NH2 | mutant L155S, 37°C, pH 7.0 | Bacillus thermoproteolyticus |