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Literature summary for 3.4.24.27 extracted from
- Inhibitory effects of alcohols on thermolysin activity as examined using a fluorescent substrate (2002), J. Biochem., 132, 945-951.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| three times crystallized and lyophilized preparation, Lot T5CB491 | Bacillus thermoproteolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-butanol | - |
Bacillus thermoproteolyticus |  |
| 1-Pentanol | - |
Bacillus thermoproteolyticus | |
| 1-propanol | - |
Bacillus thermoproteolyticus | |
| 2-butanol | - |
Bacillus thermoproteolyticus | |
| 2-methyl-1-propanol | - |
Bacillus thermoproteolyticus | |
| 2-propanol | - |
Bacillus thermoproteolyticus | |
| ethanol | - |
Bacillus thermoproteolyticus | |
| methanol | - |
Bacillus thermoproteolyticus | |
| tert-amyl alcohol | - |
Bacillus thermoproteolyticus | |
| tert-amylalcohol | - |
Bacillus thermoproteolyticus | |
| tert-butyl alcohol | - |
Bacillus thermoproteolyticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 4 calcium ions required for structural stability | Bacillus thermoproteolyticus | |
| NaCl | induces activation | Bacillus thermoproteolyticus | |
| Zn2+ | required for enzyme activity | Bacillus thermoproteolyticus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thermoproteolyticus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (7-methoxycoumarin-4-yl) acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 + H2O | fluorescent substrate | Bacillus thermoproteolyticus | ? | - |
? | |
| N-[3-(2-furyl)acryloyl]-Gly-L-Leu-NH2 + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35 | - |
1-Pentanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 38 | - |
1-propanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 40 | - |
2-butanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 41 | - |
2-methyl-1-propanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 50 | - |
1-butanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 65 | - |
1-propanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 100 | - |
ethanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 110 | - |
tert-amylalcohol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 190 | - |
tert-butyl alcohol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
| 430 | - |
methanol | pH 7.5, 25°C | Bacillus thermoproteolyticus | |
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