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Literature summary for 3.4.24.26 extracted from
- Anti-virulence strategy against the multidrug-resistant bacterial pathogen Pseudomonas aeruginosa pseudolysin (elastase B) as a potential druggable target (2019), Curr. Protein Pept. Sci., 20, 471-487 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme is a promising target for the development of new anti-virulence compounds | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, complete inhibition | Pseudomonas aeruginosa |  |
| 1-(biphenyl-4-ylmethyl)-3-hydroxy-2-methylpyridine-4(1H)-thione | - |
Pseudomonas aeruginosa | |
| 5-fluorouracil | - |
Pseudomonas aeruginosa | |
| Baicalin | - |
Pseudomonas aeruginosa | |
| dehydroleucodine | - |
Pseudomonas aeruginosa | |
| EDTA | - |
Pseudomonas aeruginosa | |
| EGTA | - |
Pseudomonas aeruginosa | |
| eriodictyol | - |
Pseudomonas aeruginosa | |
| eugenol | - |
Pseudomonas aeruginosa | |
| HS-CH2-CO-Phe-Tyr-NH2 | at 0.2 mM and 0.025 mM inhibits the degradation of the pseudolysin natural substrates nucleoside diphosphate kinase and IgG, respectively | Pseudomonas aeruginosa | |
| HSAc-Leu-Phe | 0.1 mM, inhibits 97% of the degradation of azocasein and elastin substrates by pseudolysin | Pseudomonas aeruginosa | |
| HSAc-Phe-Leu | 0.1 mM, inhibits 97% of the degradation of azocasein and elastin substrates by pseudolysin | Pseudomonas aeruginosa | |
| N-aryl mercaptoacetamide | - |
Pseudomonas aeruginosa | |
| N-mercaptoacetyl-Phe-Tyr-amide | - |
Pseudomonas aeruginosa | |
| naringenin | - |
Pseudomonas aeruginosa | |
| phosphoramidon | powerful inhibition action on pseudolysin | Pseudomonas aeruginosa | |
| taxifolin | - |
Pseudomonas aeruginosa | |
| vitexin | - |
Pseudomonas aeruginosa | |
| zingerone | - |
Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | most abundant peptidase found in pseudomonal secretions, which contributes to the invasiveness of Pseudomonas aeruginosa due to its ability to cleave several extracellular matrix proteins and to disrupt the basolateral intercellular functions present in the host tissues | Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc-dependent metallopeptidase | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
- |
Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| IgG + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O | - |
Pseudomonas aeruginosa | N-succinyl-Ala-Ala-Ala + 4-nitroaniline | - |
? | |
| nucleoside diphosphate kinase + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| elastase B | - |
Pseudomonas aeruginosa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000041 | - |
N-mercaptoacetyl-Phe-Tyr-amide | pH and temperature not specified in the publication | Pseudomonas aeruginosa | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0000059 | - |
pH and temperature not specified in the publication | Pseudomonas aeruginosa | N-aryl mercaptoacetamide | |
| 0.00273 | - |
pH and temperature not specified in the publication | Pseudomonas aeruginosa | 1-(biphenyl-4-ylmethyl)-3-hydroxy-2-methylpyridine-4(1H)-thione | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme outstands as a pivotal virulence attribute during the infectious process, playing multifunctional roles in different aspects of the pathogen-host interaction. Most abundant peptidase found in pseudomonal secretions, which contributes to the invasiveness of Pseudomonas aeruginosa due to its ability to cleave several extracellular matrix proteins and to disrupt the basolateral intercellular functions present in the host tissues. Pseudolysin makes Pseudomonas aeruginosa able to overcome host defenses by the hydrolysis of many immunologically relevant molecules, including antibodies and complement components | Pseudomonas aeruginosa |
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