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Literature summary for 3.4.24.26 extracted from

  • Andrejko, M.; Mizerska-Dudka, M.
    Elastase B of Pseudomonas aeruginosa stimulates the humoral immune response in the greater wax moth, Galleria mellonella (2011), J. Invertebr. Pathol., 107, 16-26.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Pseudomonas aeruginosa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metalloprotease Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
gene lasB
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from cell culture medium by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and cation exchange chromatography Pseudomonas aeruginosa

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
elastin Congo red + H2O
-
Pseudomonas aeruginosa ?
-
?

Synonyms

Synonyms Comment Organism
elastase B
-
Pseudomonas aeruginosa
LasB
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function elastase B of Pseudomonas aeruginosa stimulates the humoral immune response in larvae of the greater wax moth, Galleria mellonella. The insects exhibit increased antibacterial activity with appearance of antimicrobial peptides and a higher level of lysozyme in cell-free hemolymph Pseudomonas aeruginosa