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Literature summary for 3.4.24.26 extracted from
- Purification and characterization of a solvent stable protease from Pseudomonas aeruginosa PseA (2005), J. Chromatogr. A, 1069, 155-161.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 5 mM, 0% residual activity | Pseudomonas aeruginosa |  |
| 1,4-dithiothreitol | 5 mM, 0% residual activity | Pseudomonas aeruginosa | |
| Ca2+ | 5 mM, 54% residual activity | Pseudomonas aeruginosa | |
| cetyltrimethylammonium bromide | 0.1%, 53% residual activity | Pseudomonas aeruginosa | |
| Cu2+ | 5 mM, 21% residual activity | Pseudomonas aeruginosa | |
| EDTA | 5 mM, 25% residual activity | Pseudomonas aeruginosa | |
| Mg2+ | 5 mM, 56% residual activity | Pseudomonas aeruginosa | |
| Mn2+ | 5 mM, 40% residual activity | Pseudomonas aeruginosa | |
| Ni2+ | 5 mM, 12% residual activity | Pseudomonas aeruginosa | |
| sodium dodecylsulfate | 0.1%, 61% residual activity | Pseudomonas aeruginosa | |
| Zn2+ | 5 mM, 63% residual activity | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
casein | Km-value is 2.7 mg/ml | Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Pseudomonas aeruginosa |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| benzene | 25% v/v, 30°C, stable for at least 72 h | Pseudomonas aeruginosa |
| cyclohexane | 25% v/v, 30°C, stable for at least 72 h | Pseudomonas aeruginosa |
| hexane | 25% v/v, 30°C, stable for at least 72 h | Pseudomonas aeruginosa |
| toluene | 25% v/v, 30°C, stable for at least 72 h | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 99610 | - |
60°C, pH 8.0 | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, no loss of activity | Pseudomonas aeruginosa |
| 65 | - |
10 min, 33% loss of activity | Pseudomonas aeruginosa |
| 70 | - |
10 min, 80% loss of activity | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Pseudomonas aeruginosa |
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