Application | Comment | Organism |
---|---|---|
medicine | the protective activity of elastase mutants against Pseudomonas infections may be useful in producing vaccines | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression of mutants E141D, E141Q, E141G, H223G, H223E, and H223L in Escherichia coli JM109 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E141D | no activity | Pseudomonas aeruginosa |
E141G | no activity | Pseudomonas aeruginosa |
E141Q | no activity | Pseudomonas aeruginosa |
H223E | no activity | Pseudomonas aeruginosa |
H223G | no activity | Pseudomonas aeruginosa |
H223L | no activity | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | mutants E141D, E141Q, E141G, H223G, H223E, and H223L | Pseudomonas aeruginosa | 16020 | - |
periplasm | wild-type | Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE, mature elastase | Pseudomonas aeruginosa |
51000 | - |
x * 51000, SDS-PAGE, precursor form of elastase | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P14756 | IFO 3455 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | 37°C, pH 8.0 | Pseudomonas aeruginosa | ? | - |
? | |
elastin Congo red + H2O | pH 8.0 | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE, mature elastase | Pseudomonas aeruginosa |
? | x * 51000, SDS-PAGE, precursor form of elastase | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
Pseudomonas aeruginosa elastase | - |
Pseudomonas aeruginosa |