Literature summary for 3.4.24.25 extracted from

Sonoda, H.; Sugimura, A.
Extracellular production of active vibriolysin engineered by random mutagenesis in Escherichia coli (2008), Protein Expr. Purif., 62, 153-159.

Search for more literature for 3.4.24.25

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Vibrio proteolyticus

Protein Variants

Protein Variants	Comment	Organism
additional information	isolation of a vibriolysin mutant by in vivo random mutagenensis. contrary to wild-type, the mutant is expressed as active mature vibriolysin in Escherichia coli. The N-terminal propeptide of the engineered enzyme is processed and degraded, confirming that the propeptide inhibits the mature enzyme. Two mutations result in the substitution of stop codon for Trp at position 11 in the signal peptide and of Val for Ala at position 183 in the N-terminal propeptide	Vibrio proteolyticus

Organism

Organism	UniProt	Comment	Textmining
Vibrio proteolyticus	Q00971	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	enzyme is synthesized as a preproenzyme. The propeptide inhibits the mature enzyme	Vibrio proteolyticus

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Vibrio proteolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1736	-	recombinant enzyme, 37°C, pH 7.4	Vibrio proteolyticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
azocasein + H2O	-	Vibrio proteolyticus	?	-	?

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Release 2023.1 (January 2023)