General Stability | Organism |
---|---|
Lyophilization and reconstitution results in approximately 20% loss of activity | Vibrio proteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
Furylacryloyl-Gly-Leu-NH2 | - |
Vibrio proteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Calcium | contains 1.7 mol of calcium per mol of enzyme, after 48 h of dialysis, not essential for activity, Ca2+ likely plays a structural role | Vibrio proteolyticus | |
Zinc | essential for activity | Vibrio proteolyticus | |
Zinc | enzyme contains 1 mol of zinc per mol of enzyme | Vibrio proteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34800 | - |
Aeromonas proteolytica, sedimentation equilibrium, sedimentation velocity-diffusion experiments | Vibrio proteolyticus |
35000 | - |
1 * 35000 (about), Aeromonas proteolytica, sedimentation equilibrium in presence of guanidine-HCl | Vibrio proteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio proteolyticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Vibrio proteolyticus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Vibrio proteolyticus |
Storage Stability | Organism |
---|---|
-20°C, Tricine or Tris buffer, stable for several years | Vibrio proteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
furylacryloyl-Gly-Leu-NH2 + H2O | - |
Vibrio proteolyticus | furylacryloyl-Gly + Leu-NH2 | - |
? | |
Gelatin + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
additional information | preference for bonds of the type X-Phe, X-Leu, X-Tyr to which a hydrophobic residue contributes the amino group, and whereas it is essential that the NH2-terminal position of dipeptide substrates be blocked, the enzyme hydrolyzes substrates with free carboxyl groups | Vibrio proteolyticus | ? | - |
? | |
Urea-denatured hemoglobin + H2O | - |
Vibrio proteolyticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000 (about), Aeromonas proteolytica, sedimentation equilibrium in presence of guanidine-HCl | Vibrio proteolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
or 37°C, assay at | Vibrio proteolyticus |
37 | - |
or 25°C, assay at | Vibrio proteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Vibrio proteolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | 10°C, stable for more than 1 week | Vibrio proteolyticus |