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Literature summary for 3.4.24.24 extracted from

  • Goncalves, A.; Meschiari, C.; Stetler-Stevenson, W.; Nonato, M.; Alves, C.; Espreafico, E.; Gerlach, R.
    Expression of soluble and functional full-length human matrix metalloproteinase-2 in Escherichia coli (2012), J. Biotechnol., 157, 20-24.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.24

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of soluble and functional full-length enzyme from plasmid pET5a-MMP-2 in the cytoplasm of Escherichia coli strain BL21(DE3)/pLysS, subcloning in Escherichia coli strain DH5alpha, method development and evaluation, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted as a zymogen Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 x * 72000, zymogen, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gelatin + H2O Homo sapiens
-
 ?
-
 ?
additional information Homo sapiens the proteinase has the capacity to degrade several extracellular matrix components such as type IV collagens as well as all nonfibrillar collagens, laminin, fibronectin, casein, elastin, and even fibrillar collagens ?
-
 ?
Type IV collagen + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P08253
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme is secreted as a zymogen and requires activation by cleavage of its propeptide Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble enzyme from Escherichia coli cell-free extract by gelatin affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
Gelatin + H2O fluorescent substrate for use in the assay Homo sapiens ?
-
 ?
additional information the proteinase has the capacity to degrade several extracellular matrix components such as type IV collagens as well as all nonfibrillar collagens, laminin, fibronectin, casein, elastin, and even fibrillar collagens Homo sapiens ?
-
 ?
Type IV collagen + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 72000, zymogen, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
matrix metalloproteinase-2
-
 Homo sapiens
MMP-2
-
 Homo sapiens
Type IV collagenase
-
 Homo sapiens