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Literature summary for 3.4.24.24 extracted from

  • Higashi, S.; Miyazaki, K.
    Identification of amino acid residues of the matrix metalloproteinase-2 essential for its selective inhibition by beta-amyloid precursor protein-derived inhibitor (2008), J. Biol. Chem., 283, 10068-10078.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged MMP-2 in Escherichia coli strain DH5alpha in inclusion bodies Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of MMP-2 truncation mutants overview, an MMP-2 mutant, with deleted hemopexin-like domain and three fibronectin-like type II domains, and native MMP-2 show similar affinities for APP-IP, chimeric proteases, consisting of various parts of the MMP-2 catalytic domain and those of MMP-7 or MMP-9, show that Ala88 and Gly94 in the non-prime side and Tyr145 and Thr146 in the prime side of the substrate-binding cleft of MMP-2 contribute separately to the selective inhibition bei APP-PI, mutational interaction analysis, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
extracellular domain of beta-amyloid peptide the extracellular domain of beta-amyloid precursor protein contains an inhibitor against MMP-2, the inhibitor is localized within the ISYGNDALMP sequence of APP, overview Homo sapiens
ISYGNDALMP APP-derived inhibitory peptide, APP-IP, selective inhibition of MMP-2, An MMP-2 mutant, with deleted hemopexin-like domain and three fibronectin-like type II domains, and native MMP-2 showed similar affinities for APP-IP, suggesting that only the catalytic domain of MMP-2 is essential for the interaction, mutational interaction analysis, overview, inhibition of recombinant wild-type enzyme and truncated enzyme mutants, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
solubilized, refolded recombinant GST-tagged MMP-2 by glutathione affinity chromatography, removal of the tag Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli inclusion bodies by solubilization of the inclusion bodies with guanidine-HCl followed by refolding by rapid dilution method Homo sapiens

Synonyms

Synonyms Comment Organism
gelatinase A
-
Homo sapiens
matrix metalloproteinase-2
-
Homo sapiens
MMP-2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
-
Homo sapiens additional information
0.0073 0.015 pH 7.5, 37°C, recombinant fragments of MMP-2 Homo sapiens ISYGNDALMP