Cloned (Comment) | Organism |
---|---|
expression of GST-tagged MMP-2 in Escherichia coli strain DH5alpha in inclusion bodies | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of MMP-2 truncation mutants overview, an MMP-2 mutant, with deleted hemopexin-like domain and three fibronectin-like type II domains, and native MMP-2 show similar affinities for APP-IP, chimeric proteases, consisting of various parts of the MMP-2 catalytic domain and those of MMP-7 or MMP-9, show that Ala88 and Gly94 in the non-prime side and Tyr145 and Thr146 in the prime side of the substrate-binding cleft of MMP-2 contribute separately to the selective inhibition bei APP-PI, mutational interaction analysis, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
extracellular domain of beta-amyloid peptide | the extracellular domain of beta-amyloid precursor protein contains an inhibitor against MMP-2, the inhibitor is localized within the ISYGNDALMP sequence of APP, overview | Homo sapiens | |
ISYGNDALMP | APP-derived inhibitory peptide, APP-IP, selective inhibition of MMP-2, An MMP-2 mutant, with deleted hemopexin-like domain and three fibronectin-like type II domains, and native MMP-2 showed similar affinities for APP-IP, suggesting that only the catalytic domain of MMP-2 is essential for the interaction, mutational interaction analysis, overview, inhibition of recombinant wild-type enzyme and truncated enzyme mutants, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
solubilized, refolded recombinant GST-tagged MMP-2 by glutathione affinity chromatography, removal of the tag | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli inclusion bodies by solubilization of the inclusion bodies with guanidine-HCl followed by refolding by rapid dilution method | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
gelatinase A | - |
Homo sapiens |
matrix metalloproteinase-2 | - |
Homo sapiens |
MMP-2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
- |
Homo sapiens | additional information | |
0.0073 | 0.015 | pH 7.5, 37°C, recombinant fragments of MMP-2 | Homo sapiens | ISYGNDALMP |