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Literature summary for 3.4.24.24 extracted from

  • Chen, E.I.; Li, W.; Godzik, A.; Howard, E.W.; Smith, J.W.
    A residue in the S2 subsite controls substrate selectivity of matrix metalloproteinase-2 and matrix metalloproteinase-9 (2003), J. Biol. Chem., 278, 17158-17163.
    View publication on PubMed
Search for more literature for 3.4.24.24

Cloned(Commentary)

Cloned (Comment) Organism
cDNA of the mutated enzyme used to transfect HEK 293 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
E412D site-directed mutagenesis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 peptide A13R 37°C, mutant E412D Homo sapiens
0.2
-
 peptide B74 37°C, mutant E412D Homo sapiens
0.4
-
 peptide A13R 37°C Homo sapiens
0.9
-
 peptide C9 37°C, mutant E412D Homo sapiens
1.2
-
 peptide A3 37°C, mutant E412D Homo sapiens
1.3
-
 peptide A13 37°C Homo sapiens
2.2
-
 peptide A34 37°C Homo sapiens
2.2
-
 peptide B74 37°C Homo sapiens
2.5
-
 peptide A13 37°C, mutant E412D Homo sapiens
3.6
-
 peptide A3 37°C Homo sapiens
4.3
-
 peptide A34 37°C, mutant E412D Homo sapiens
4.4
-
 peptide C9 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P08253 human
-

Purification (Commentary)

Purification (Comment) Organism
catalytic domain of mutated enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ephB1 + H2O
-
 Homo sapiens ?
-
 ?
peptide A13 + H2O
-
 Homo sapiens ?
-
 ?
peptide A13R + H2O
-
 Homo sapiens ?
-
 ?
peptide A3 + H2O
-
 Homo sapiens ?
-
 ?
peptide A34 + H2O
-
 Homo sapiens ?
-
 ?
peptide B74 + H2O
-
 Homo sapiens ?
-
 ?
peptide C15 + H2O non-selective peptide substrate Homo sapiens ?
-
 ?
peptide C9 + H2O
-
 Homo sapiens ?
-
 ?
peptide m1A11 + H2O non-selective peptide substrate Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
gelatinase A
-
 Homo sapiens
metrix metalloproteinase-2
-
 Homo sapiens
MMP-2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 peptide A13R 37°C Homo sapiens
17
-
 peptide B74 37°C, mutant E412D Homo sapiens
18
-
 peptide A13R 37°C, mutant E412D Homo sapiens
26
-
 peptide C9 37°C, mutant E412D Homo sapiens
31
-
 peptide A13 37°C, mutant E412D Homo sapiens
60
-
 peptide A34 37°C, mutant E412D Homo sapiens
73
-
 peptide A3 37°C, mutant E412D Homo sapiens
105
-
 peptide A13 37°C Homo sapiens
202
-
 peptide A34 37°C Homo sapiens
400
-
 peptide A3 37°C Homo sapiens
622
-
 peptide B74 37°C Homo sapiens
740
-
 peptide C9 37°C Homo sapiens