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Literature summary for 3.4.24.24 extracted from

  • Ye, Q.Z.; Johnson, L.L.; Yu, A.E.; Hupe, D.
    Reconstructed 19 kDa catalytic domain of gelatinase A is an active proteinase (1995), Biochemistry, 34, 4702-4708.
    View publication on PubMed
Search for more literature for 3.4.24.24

Inhibitors

Inhibitors Comment Organism Structure
Stromelysin catalytic domain inhibitors gelatinase A synthetic 19000 MW catalytic domain Homo sapiens
Zn2+ required, synthetic 19000 MW catalytic domain, inhibition at high concentration Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.134
-
 Acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester gelatinase A synthetic 19000 MW catalytic domain Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, gelatinase A synthetic 19000 MW catalytic domain Homo sapiens
Zn2+ required, gelatinase A synthetic 19000 MW catalytic domain, inhibition at high concentration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 synthetic gene in which two polypeptide fragments of the catalytic domain are joined with the deletion of the 19000 MW fibronectin-like insert of the catalytic domain, expressed in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-Dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Ala-NH2 + H2O
-
 Homo sapiens ?
-
 ?
Acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester + H2O
-
 Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
 Acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester, gelatinase A synthetic 19000 MW catalytic domain Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 gelatinase A synthetic 19000 MW catalytic domain Homo sapiens