Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of mutant MMP-7(29,33,51,55/M2)DELTAC3, a variant of MMP-7 lacking affinity for cell surface cholesterol sulfate (CS) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibits MMP-7-induced cell aggregation | Homo sapiens | |
methyl-beta-cyclodextrin | inhibits release of the HAI-1 fragment by MMP-7-catalyzed cleavage | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | extracellular MMP-7 binds to cell-surface cholesterol sulfate (CS) and acts as a membrane-associated protease | Homo sapiens | 9986 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal ions are required for the MMP-7-induced cell aggregation | Homo sapiens | |
Zn2+ | a zinc metalloproteinase | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hepatocyte growth factor activator inhibitor type 1 + H2O | Homo sapiens | membrane-bound, biotinylated Kunitz-type inhibitor HAI-1, cell-bound MMP-7 cleaves HAI-1 mainly between Gly451 and Leu452 and thereby releases the extracellular region as soluble HAI-1 (sHAI-1, comprising amino acids 141-249). The peptide bonds corresponding to Gly375-Phe376 and Glu378-Leu379 in HAI-1 are slightly susceptible to MMP-7 cleavage | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09237 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
carcinoma cell | - |
Homo sapiens | - |
Colo-201 cell | - |
Homo sapiens | - |
colonic cancer cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hepatocyte growth factor activator inhibitor type 1 + H2O | membrane-bound, biotinylated Kunitz-type inhibitor HAI-1, cell-bound MMP-7 cleaves HAI-1 mainly between Gly451 and Leu452 and thereby releases the extracellular region as soluble HAI-1 (sHAI-1, comprising amino acids 141-249). The peptide bonds corresponding to Gly375-Phe376 and Glu378-Leu379 in HAI-1 are slightly susceptible to MMP-7 cleavage | Homo sapiens | ? | - |
? | |
hepatocyte growth factor activator inhibitor type 1 + H2O | membrane-bound, biotinylated Kunitz-type inhibitor HAI-1 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Matrix metalloproteinase-7 | - |
Homo sapiens |
MMP-7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | MMP-7-induced cancer cell aggregation is an important mechanism in cancer metastasis, and the soluble fragment of hepatocyte growth factor activator inhibitor type 1 (sHAI-1) is an essential component of MMP-7-induced stimulation of cancer metastasis | Homo sapiens |
physiological function | matrix metalloproteinase-7 (MMP-7) plays important roles in tumor progression and metastasis. Treatment of human colon carcinoma cells with active MMP-7 in vitro induces cell aggregation by cleaving cell-surface proteins. MMP-7 binds to colon cancer cells via cell surface-bound cholesterol sulfate and induces significant cell aggregation by cleaving cell-surface protein(s). These aggregated cells exhibit a dramatically enhanced metastatic potential. MMP-7 induces homotypic tumor cell aggregation via proteolytic cleavage of the membrane-bound Kunitz-type inhibitor HAI-1. Cell-bound MMP-7 cleaves HAI-1 mainly between Gly451 and Leu452 and thereby releases the extracellular region as soluble HAI-1 (sHAI-1). sHAI-1 can induce cancer cell aggregation. MMP-7-catalyzed generation of sHAI-1 requires cholesterol sulfate (CS). Binding of MMP-7 to CS is important for cleavage of HAI-1 localized in raft region. The MMP-7-induced cell aggregation of colon carcinoma cells consists of two steps, initial loose cell aggregation is secondly converted to tight cell aggregation. Region of HAI-1 corresponding to amino acid residues 141-249 is essential for cell aggregation-inducing activity of MMP-7 | Homo sapiens |