Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.23 extracted from

  • Samukange, V.; Yasukawa, K.; Inouye, K.
    Effects of heparin and cholesterol sulfate on the activity and stability of human matrix metalloproteinase 7 (2014), Biosci. Biotechnol. Biochem., 78, 41-48.
    View publication on PubMed
Search for more literature for 3.4.24.23

Activating Compound

Activating Compound Comment Organism Structure
heparin porcine intestinal heparin, increases activity by decreasing the Km value, increases the enzyme's thermostability Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cholesterol sulfate non-competitive inhibition, decreases the enzyme's thermostability, inhibition mechanism, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 (7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, with 0.05 mM heparin Homo sapiens
0.057
-
 (7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, without heparin Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09237
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme is matured by proteolytic cleavage, the mature MMP-7 comprises Tyr78-Lys250 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant solubilized and refolded enzyme from Escherichia coli strain BL21(DE3) inclusion bodies by ammonium sulfate fractionation and heparin affinity chromatography Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
recombinant enzyme, solubilization from Escherichia coli strain BL21(DE3) inclusion bodies by 6 M guanidine HCl, and refolded with 1 M L-arginine Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 + H2O
-
 Homo sapiens (7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly + L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2
-
 ?

Synonyms

Synonyms Comment Organism
Matrix metalloproteinase 7
-
 Homo sapiens
MMP-7
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 in thermal incubation of the enzyme at 50-70°C, heparin increases the relative activity (the ratio of kcat/Km of MMP-7 with incubation to that without it), while cholesterol sulfate decreases the relative activity Homo sapiens
50 60 purified enzyme with or without heparin and cholesterol sulfate, 10 min, 10-40% activity remaining, overview Homo sapiens
70
-
 purified enzyme, inactivation within 10 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.011
-
 Cholesterol sulfate pH 7.5, 25°C Homo sapiens