Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cholesterol sulfate | selectively alters substrate preference of matrix metalloproteinase-7 and promotes degradations of pericellular laminin-332 and fibronectin. Degradation of laminin-332 (laminin-5) catalyzed by MMP-7 is accelerated dramatically in the presence of cholesterol sulfate, whereas the sulfated lipid inhibits the degradation of casein catalyzed by the protease. Cholesterol sulfate facilitates the proteolyses by cross-linking MMP-7 to its substrates, mechanism, overview | Homo sapiens | |
additional information | cholesterol, dehydroepiandrosterone sulfate, and heparin do not affect MMP-7-catalyzed degradations of fibronactin and laminin-332 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | MMP-7 tethered to cancer cell surface via cholesterol sulfate degrades fibronectin and laminin-332 coated on a culture plate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-{(2R)-2-[2-(hydroxyamino)-2-oxoethyl]-4-methylpentanoyl}-3-(naphthalen-2-yl)-L-alanyl-N-(2-aminoethyl)-L-alaninamide | i.e. TAPI-1, a hydroxamate-based metalloproteinase inhibitor | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | the enzyme is secreted | Homo sapiens | 9986 | - |
extracellular | the enzyme is secreted | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metalloprotease | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibronectin + H2O | Homo sapiens | also MMP-7-catalyzed cleavages of chymotryptic fragments of fibronectin by cell-bound MMP-7, overview | ? | - |
? | |
laminin-332 + H2O | Homo sapiens | pericellular substrate | ? | - |
? | |
additional information | Homo sapiens | binding of MMP-7 to EJ-1 cell surface | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Colo-201 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Leu-[N-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-Ala-Arg amide + H2O | - |
Homo sapiens | ? | - |
? | |
Fibronectin + H2O | also MMP-7-catalyzed cleavages of chymotryptic fragments of fibronectin by cell-bound MMP-7, overview | Homo sapiens | ? | - |
? | |
Fibronectin + H2O | pericellular substrate | Homo sapiens | ? | - |
? | |
kappa-casein + H2O | - |
Homo sapiens | ? | - |
? | |
laminin-332 + H2O | - |
Homo sapiens | ? | analysis of peptide fragment degradation products | ? | |
laminin-332 + H2O | pericellular substrate | Homo sapiens | ? | - |
? | |
additional information | binding of MMP-7 to EJ-1 cell surface | Homo sapiens | ? | - |
? | |
additional information | immunohistochemic analysis of peptide fragment degradation products | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Matrix metalloproteinase-7 | - |
Homo sapiens |
MMP-7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | binding of MMP-7 to cell surface cholesterol sulfate is essential for the cell membrane-associated proteolytic action of MMP-7 thereby ECM, thereby. Degradation of laminin-332 (laminin-5) catalyzed by MMP-7 is accelerated dramatically in the presence of cholesterol sulfate, whereas the sulfated lipid inhibits the degradation of casein catalyzed by the protease. Cholesterol sulfate facilitates the proteolyses by cross-linking MMP-7 to its substrates, mechanism, overview | Homo sapiens |