Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant matrilysins in Escherichia coli strain BL21 in inclusion bodies | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
E-cadherin + H2O | Homo sapiens | matrilysin cleaves E-cadherin in its juxtamembrane stalk releasing the entire ectodomain | modified E-cadherin + E-cadherin ectodomain | - |
? | |
fibronactin + H2O | Homo sapiens | - |
fibronectin peptide fragments | MWs of 30 to 175 kDa | ? | |
pro-alpha-defensin-1 + H2O | Homo sapiens | i.e. procryptdins | alpha-defensin-1 + alpha-defensin-1 propeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | promatrilysin shows autolytic activation, promatrilysin activation is regulated by sulfated glycosaminoglycans, overview. Presence of chondroitin-4,6-sulfate markedly accelerates activation of promatrilysin. Chondroitin-4,6-sulfate and heparin also markedly accelerates the ability of active wild-type matrilysin to cleave inactive promatrilysin | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant matrilysins, after solubilization from Escherichia coli strain BL21 inclusion bodies, by cation exchange chromatography and gel filtration | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
recombinant wild-type and mutant matrilysins from Escherichia coli strain BL21 inclusion bodies dialyzed in 8 M urea containing 50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, 0.1 mM zinc acetate, 0.05% Brij-35, 0.02% NaN3, and 0.01% Triton X-100, followed by gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxy-coumarin-4-yl)acetyl-Ala-Pro-Lys-2,4-dinitrophenol + H2O | - |
Homo sapiens | ? | - |
? | |
E-cadherin + H2O | matrilysin cleaves E-cadherin in its juxtamembrane stalk releasing the entire ectodomain | Homo sapiens | modified E-cadherin + E-cadherin ectodomain | - |
? | |
fibronactin + H2O | - |
Homo sapiens | fibronectin peptide fragments | MWs of 30 to 175 kDa | ? | |
pro-alpha-defensin-1 + H2O | i.e. procryptdins | Homo sapiens | alpha-defensin-1 + alpha-defensin-1 propeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MMP7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |