Cloned (Comment) | Organism |
---|---|
sequence comparisons and phylogenetic tree, recombinant expression of SUMO-His6-tagged enzyme in Escherichia coli strain SHuffle T7 Express LysY | Loxosceles intermedia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Loxosceles intermedia | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc metalloproteinase. Molecular modeling of LALP3 reveals that the isoform contains the zinc binding and Met-turn motifs, forming the active site, as has been observed in astacins. Homology three-dimensional structure modeling and structural analysis of LALP3 | Loxosceles intermedia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loxosceles intermedia | C9D7R3 | wild spiders caught in Lapa City, Parana, Brazil | - |
Purification (Comment) | Organism |
---|---|
recombinant SUMO-His6-tagged enzyme from Escherichia coli strain SHuffle T7 Express LysY by nickel affinity chromatography | Loxosceles intermedia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Loxosceles intermedia | - |
Subunits | Comment | Organism |
---|---|---|
More | peptide mapping, LC-MS/MS analysis | Loxosceles intermedia |
Synonyms | Comment | Organism |
---|---|---|
astacin-like metalloprotease | - |
Loxosceles intermedia |
astacin-like metalloprotease toxin 3 | UniProt | Loxosceles intermedia |
astacin-Like protease 3 | - |
Loxosceles intermedia |
LALP3 | - |
Loxosceles intermedia |
toxin 3 | - |
Loxosceles intermedia |
General Information | Comment | Organism |
---|---|---|
additional information | molecular modeling of LALP3 reveals that this isoform contains the zinc binding and Met-turn motifs, forming the active site, as has been observed in astacins. LALP3 epitopes are recognized by polyclonal antibodies raised against Loxosceles intermedia whole venom and LALP1 in a conformation-dependent manner | Loxosceles intermedia |