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Literature summary for 3.4.24.21 extracted from

  • Morgon, A.; Belisario-Ferrari, M.; Trevisan-Silva, D.; Meissner, G.; Vuitika, L.; Marin, B.; Tashima, A.; Gremski, L.; Gremski, W.; Senff-Ribeiro, A.; Veiga, S.; Chaim, O.
    Expression and immunological cross-reactivity of LALP3, a novel astacin-like metalloprotease from brown spider (Loxosceles intermedia) venom (2016), Biochimie, 128-129, 8-19 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
sequence comparisons and phylogenetic tree, recombinant expression of SUMO-His6-tagged enzyme in Escherichia coli strain SHuffle T7 Express LysY Loxosceles intermedia

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Loxosceles intermedia
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc metalloproteinase. Molecular modeling of LALP3 reveals that the isoform contains the zinc binding and Met-turn motifs, forming the active site, as has been observed in astacins. Homology three-dimensional structure modeling and structural analysis of LALP3 Loxosceles intermedia

Organism

Organism UniProt Comment Textmining
Loxosceles intermedia C9D7R3 wild spiders caught in Lapa City, Parana, Brazil
-

Purification (Commentary)

Purification (Comment) Organism
recombinant SUMO-His6-tagged enzyme from Escherichia coli strain SHuffle T7 Express LysY by nickel affinity chromatography Loxosceles intermedia

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Loxosceles intermedia
-

Subunits

Subunits Comment Organism
More peptide mapping, LC-MS/MS analysis Loxosceles intermedia

Synonyms

Synonyms Comment Organism
astacin-like metalloprotease
-
Loxosceles intermedia
astacin-like metalloprotease toxin 3 UniProt Loxosceles intermedia
astacin-Like protease 3
-
Loxosceles intermedia
LALP3
-
Loxosceles intermedia
toxin 3
-
Loxosceles intermedia

General Information

General Information Comment Organism
additional information molecular modeling of LALP3 reveals that this isoform contains the zinc binding and Met-turn motifs, forming the active site, as has been observed in astacins. LALP3 epitopes are recognized by polyclonal antibodies raised against Loxosceles intermedia whole venom and LALP1 in a conformation-dependent manner Loxosceles intermedia