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Literature summary for 3.4.24.18 extracted from
- N-Linked oligosaccharides on the meprin A metalloprotease are important for secretion and enzymatic activity, but not for apical targeting (2000), J. Biol. Chem., 275, 25577-25584.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Trypsin | activated by limited trypsin digestion | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pcDNAI/Amp plasmid containing full-length wild-type meprin alpha-subunit cDNA transfected into MDCK cells and human embryonic kidney 293 cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N152Q | site-directed mutagenesis | Mus musculus |
| N234Q | site-directed mutagenesis | Mus musculus |
| N270Q | site-directed mutagenesis | Mus musculus |
| N330Q | site-directed mutagenesis | Mus musculus |
| N41Q | site-directed mutagenesis | Mus musculus |
| N426Q | site-directed mutagenesis | Mus musculus |
| N452Q | site-directed mutagenesis | Mus musculus |
| N546Q | site-directed mutagenesis | Mus musculus |
| N553Q | site-directed mutagenesis | Mus musculus |
| N614Q | site-directed mutagenesis | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Mus musculus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc metalloendopeptidase | Mus musculus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
SDS-PAGE | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
mouse | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.9 | - |
mutant N152Q | Mus musculus |
| 1.4 | - |
mutant N426Q and N41Q | Mus musculus |
| 1.6 | - |
mutant N614Q | Mus musculus |
| 2 | - |
mutant N270Q | Mus musculus |
| 2.1 | - |
mutant N330Q | Mus musculus |
| 2.2 | - |
mutant N546Q | Mus musculus |
| 2.4 | - |
mutant N234Q | Mus musculus |
| 3.2 | - |
wild-type | Mus musculus |
| 3.3 | - |
mutant N553Q | Mus musculus |
| 5.9 | - |
mutant N452Q | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| meprin A metalloprotease | - |
Mus musculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
wild-type enzyme retains 55% activity after 20 min, similar results for the mutants N41Q, N330Q, N426Q, N452Q, N546Q, and N553Q, mutants N234Q, N270Q, and N614Q retains 10% activity after 20 min, N152Q retains 6% activity after 5 min | Mus musculus |
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