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Literature summary for 3.4.24.18 extracted from

  • Kounnas, M.Z.; Woltz, R.L.; Gorbea, C.M.; Bond, J.S.
    Meprin-A and -B. Cell surface endopeptidases of the mouse kidney (1991), J. Biol. Chem., 266, 17350-17357.
    View publication on PubMed
Search for more literature for 3.4.24.18

Activating Compound

Activating Compound Comment Organism Structure
additional information contrary to meprin B only very little activation by trypsin treatment Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Mus musculus
cysteine
-
 Mus musculus
EDTA
-
 Mus musculus
additional information iodoacetate; L-trans-epoxysuccinyl-leucylamido-(4-guanidino)-butane (i.e. E-64); no inhibition by phosphoramidon; soybean trypsin inhibitor Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane renal brush border membrane Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc metalloendopeptidase Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition (of meprin A and B) Mus musculus
90000
-
 4 * 90000, mouse, SDS-PAGE, reducing conditions Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 male ICR
-
Mus musculus male ICR
-
 male ICR
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein complex-type glycosylation Mus musculus

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 4.6673 mg azocasein/min/mg (untreated enzyme), 7.2512 mg azocasein/min/mg (trypsin-treated enzyme) Mus musculus
0.095
-
 Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly Mus musculus
1.38
-
 oxidized insulin B-chain, trypsin-treated enzyme Mus musculus
4.54
-
 n-bradykinin Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Mus musculus fragments of azocasein
-
 ?
azocasein + H2O
-
 Mus musculus male ICR fragments of azocasein
-
 ?
bradykinin + H2O
-
 Mus musculus Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg
-
 ?
Insulin B-chain + H2O prevalent cleavage sites are Gly20-Glu21, Phe24-Phe25 and Phe25-Tyr26 Mus musculus Hydrolyzed insulin B-chain 15 different peptide fragments ?
Insulin B-chain + H2O 10 cleavage sites Mus musculus Hydrolyzed insulin B-chain 15 different peptide fragments ?
Insulin B-chain + H2O oxidized form Mus musculus Hydrolyzed insulin B-chain 15 different peptide fragments ?
Insulin B-chain + H2O prevalent cleavage sites are Gly20-Glu21, Phe24-Phe25 and Phe25-Tyr26 Mus musculus male ICR Hydrolyzed insulin B-chain 15 different peptide fragments ?
Insulin B-chain + H2O 10 cleavage sites Mus musculus male ICR Hydrolyzed insulin B-chain 15 different peptide fragments ?
Insulin B-chain + H2O oxidized form Mus musculus male ICR Hydrolyzed insulin B-chain 15 different peptide fragments ?
Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly + H2O synthetic peptide derived from insulin B-chain Mus musculus ?
-
 ?
Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly + H2O synthetic peptide derived from insulin B-chain Mus musculus male ICR ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 90000, mouse, SDS-PAGE, reducing conditions Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
 t1/2: 50 min Mus musculus