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Literature summary for 3.4.24.18 extracted from

  • Sterchi, E.E.; Naim, H.Y.; Lentze, M.J.; Hauri, H.P.; Fransen, J.A.M.
    N-Benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase: a metalloendopeptidase of the human intestinal microvillus membrane which degrades biologically active peptides (1988), Arch. Biochem. Biophys., 265, 105-118.
    View publication on PubMed

General Stability

General Stability Organism
Solubilized PPH is rather labile, immobilization by protein A-Sepharose stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Homo sapiens
2-(N-Hydroxycarboxamido)-4-methylpentanoyl-L-alanyl-glycylamide i.e. Zinkov inhibitor Homo sapiens
Ca2+ above 1 mM, metalloproteinase, does not require additional Ca2+ Homo sapiens
captopril
-
Homo sapiens
DTT
-
Homo sapiens
EDTA restored by divalent metal ions, Zn2+ being more effective than Ca2+ or Mg2+ Homo sapiens
additional information amastatin, bestatin, puromycin, N-tosyl-L-Phe chloromethyl ketone, monoclonal antibody HBB 3/716/36; iodoacetate; leupeptin; no inhibition by phosphoramidon; PMSF, pepstatin Homo sapiens
Zn2+ above 1 mM, metalloproteinase, does not require additional Zn2+ Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane apical membrane of intestinal epithelial cells Homo sapiens 16020
-
microvillus
-
Homo sapiens 5902
-

Metals/Ions

Metals/Ions Comment Organism Structure
Calcium metalloendopeptidase Homo sapiens
Calcium Ca2+ inhibits above 1 mM Homo sapiens
Zinc Zn2+ inhibits above 1 mM Homo sapiens
Zinc metalloendopeptidase Homo sapiens
Zinc zinc binding motif Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
2 * 100000, human, SDS-PAGE in the presence of DTT Homo sapiens
200000
-
human, SDS-PAGE, non-reducing conditions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
PPH
-
Homo sapiens PPH
-
PPH
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein highly glycosylated Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
from partially purified microvillar membranes Homo sapiens
immunoaffinity chromatography Homo sapiens
to near homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
intestine higher specific activity in distal ileum than in proximal duodenum Homo sapiens
-

Storage Stability

Storage Stability Organism
4°C, immobilized enzyme, 3 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O 2 cleavage sites: Tyr-Ile and Phe-His Homo sapiens Asp-Arg-Val-Tyr + Ile-His-Pro-Phe + Ile-His-Pro-Phe-His-Leu
-
?
angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleavage site: Tyr-Ile Homo sapiens Asp-Arg-Val-Tyr + Ile-His-Pro-Phe + Ile-His-Pro-Phe-His-Leu
-
?
angiotensin II + H2O cleavage site: Tyr-Ile Homo sapiens Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
?
angiotensin II + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe Homo sapiens Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
?
azocasein + H2O
-
Homo sapiens fragments of azocasein
-
?
azocasein + H2O
-
Homo sapiens PPH fragments of azocasein
-
?
benzoyl-Gly-His-Leu + H2O very poor substrate, t1/2 of more than 16 h Homo sapiens ?
-
?
benzoyl-Gly-His-Leu + H2O very poor substrate, t1/2 of more than 16 h Homo sapiens PPH ?
-
?
bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, bradykinin(1-7) and bradykinin(1-8) best substrates next to substance P Homo sapiens Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg
-
?
bradykinin + H2O one cleavage site: Phe-Ser Homo sapiens Arg-Pro-Pro-Gly + Phe-Ser-Pro-Phe-Arg
-
?
additional information hydrolysis occurs on carboxy side of aromatic residues Homo sapiens ?
-
?
additional information dansyl-(D)-Ala-Gly-4-phenylalanylglycine, [Leu]-enkephalin, [Met]-enkephalin Homo sapiens ?
-
?
additional information hydrolysis occurs on carboxy side of aromatic residues Homo sapiens PPH ?
-
?
additional information dansyl-(D)-Ala-Gly-4-phenylalanylglycine, [Leu]-enkephalin, [Met]-enkephalin Homo sapiens PPH ?
-
?
N-Benzoyl-L-tyrosyl-4-aminobenzoate + H2O very poor substrate, t1/2 of more than 16 h Homo sapiens N-Benzoyl-L-tyrosine + 4-aminobenzoate
-
?
N-Benzoyl-L-tyrosyl-4-aminobenzoate + H2O very poor substrate, t1/2 of more than 16 h Homo sapiens PPH N-Benzoyl-L-tyrosine + 4-aminobenzoate
-
?
Oxytocin + H2O poor substrate Homo sapiens Hydrolyzed oxytocin
-
?
Substance P + H2O best substrate Homo sapiens Hydrolyzed substance P
-
?

Subunits

Subunits Comment Organism
dimer 2 * 100000, human, SDS-PAGE in the presence of DTT Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
marked microheterogeneity due to glycosylation with pIs in the range of 6-6.85 Homo sapiens
7.5 8
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 8 plateau with maximal activity at pH 7.5-8 Homo sapiens