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Literature summary for 3.4.24.17 extracted from

  • Baricos, W.H.; Murphy, G.; Zhou, Y.; Nguyen, H.H.; Shah, S.V.
    Degradation of glomerular basement membrane by purified mammalian metalloproteinases (1988), Biochem. J., 254, 609-612.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.17

General Stability

General Stability Organism
Ca2+ stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Homo sapiens
EDTA
-
 Homo sapiens
additional information not: inhibitors for serine, cysteine or aspartic proteinases; phosphoramidon Homo sapiens
NEM
-
 Homo sapiens
U24522 synthetic inhibitor known to inhibit proteoglycan-degrading metalloproteinases Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly-ethylester
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 expression in Escherichia coli
-
Homo sapiens
-
 catalytic domain lacking both propeptide and C-terminal fragment
-

Purification (Commentary)

Purification (Comment) Organism
catalytic domain lacking both propeptide and C-terminal fragment Homo sapiens
expression in Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl, pH 7.6, 10 mM CaCl2, stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester + H2O
-
 Homo sapiens ?
-
 ?
Proteoglycan + H2O
-
 Homo sapiens More
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable at pH 6-9 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.12
-
 Acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly ethyl ester
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 acetyl-Pro-Leu-Gly-thioester-Leu-Leu-Gly-ethylester Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: about 20% of activity maximum, pH 7.5: about 25% of activity maximum Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 37°C, stable Homo sapiens