Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.16 extracted from

  • Wangler, N.J.; Jayaraman, S.; Zhu, R.; Mechref, Y.; Abbruscato, T.J.; Bickel, U.; Karamyan, V.T.
    Preparation and preliminary characterization of recombinant neurolysin for in vivo studies (2016), J. Biotechnol., 234, 105-115 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information catalytic activity of recombinant Nln increases over time upon incubation in fresh mouse blood. The recombinant protein easily reaches the systemiccirculation remaining both catalytically active and physically intact Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli Rattus norvegicus

General Stability

General Stability Organism
up to two cycles of freezing and thawing of the purified rNln do not significantly affect the purified recombinant enzyme's specific activity Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Rattus norvegicus
Zn2+ zinc metalloproteinase Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P42676
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, ultrafiltration, and gel filtration to homogeneity, large scale production of rNln Rattus norvegicus

Storage Stability

Storage Stability Organism
a gradual decrease of the specific activity is observed in preparations kept longer than 6 months at -80°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
Rattus norvegicus ?
-
?
bradykinin + H2O
-
Rattus norvegicus ?
-
?
neurotensin + H2O
-
Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
? x * 75000, recombinant His6-tagged enzyme, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
Nln
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Rattus norvegicus