Literature summary for 3.4.24.16 extracted from

Rioli, V.; Gozzo, F.C.; Heimann, A.S.; Linardi, A.; Krieger, J.E.; Shida, C.S.; Almeida, P.C.; Hyslop, S.; Eberlin, M.N.; Ferro, E.S.
Novel natural peptide substrates for endopeptidase 24.15, neurolysin, and angiotensin-converting enzyme (2003), J. Biol. Chem., 278, 8547-8555.

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Protein Variants

Protein Variants	Comment	Organism
E502A	complete loss of enzymatic activity	Rattus norvegicus
H473A	complete loss of enzymatic activity	Rattus norvegicus
H474A	complete loss of enzymatic activity	Rattus norvegicus
H477A	complete loss of enzymatic activity	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
angiotensin I	inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
angiotensin II	inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
bradykinin	inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
dynorphin A1-13	inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
LVVYPWTQRY	inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
PVNFKFLSH	inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
VVYPWTQRY	inhibits hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
testis	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl) + H2O	-	Rattus norvegicus	?	-	?
angiotensin I + H2O	-	Rattus norvegicus	?	-	?
angiotensin II + H2O	-	Rattus norvegicus	?	-	?
bradykinin + H2O	-	Rattus norvegicus	?	-	?
dynorphin A1-13 + H2O	-	Rattus norvegicus	?	-	?
LVVYPWTQRY + H2O	cleavage sites: LVVYP-/-W-/-T-/-Q-/-RY	Rattus norvegicus	?	-	?
PVNFKFLSH + H2O	cleavage sites: PVNF-/-K-/-F-/-LSH	Rattus norvegicus	?	-	?
VVYPWTQRY + H2O	cleavage sites: VVYPW-/-T-/-Q-/-RY	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
endopeptidase 24.16	-	Rattus norvegicus
ep24.16	-	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000022	-	dynorphin A1-13	inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
0.00201	-	LVVYPWTQRY	inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
0.00343	-	PVNFKFLSH	inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
0.00535	-	angiotensin I	inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
0.00704	-	VVYPWTQRY	inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
0.00795	-	angiotensin II	inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus
0.00811	-	bradykinin	inhibition of hydrolysis of 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-D-Lys-(2,4-dinitrophenyl)	Rattus norvegicus

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Release 2023.1 (January 2023)