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Literature summary for 3.4.24.15 extracted from

  • Icimoto, M.Y.; Ferreira, J.C.; Yokomizo, C.H.; Bim, L.V.; Marem, A.; Gilio, J.M.; Oliveira, V.; Nantes, I.L.
    Redox modulation of thimet oligopeptidase activity by hydrogen peroxide (2017), FEBS Open Bio, 7, 1037-1050 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.15

Activating Compound

Activating Compound Comment Organism Structure
H2O2
-
 Homo sapiens
H2O2 synergistic effect of antimycin A, succinate, and antimycin A plus succinate on activation by H2O2. Antimycin A and succinate stimulate the H2O2 production and increase rTOP activity Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
gene Thop1, recombinant expression of wild-type and mutant enzymes Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C246S/C248S/C253S site-directed mutagenesis, the mutations abolish the DTT and H2O2 effects on rTOP activity. The triple-mutated rTOP is not affected by DTT Rattus norvegicus

General Stability

General Stability Organism
H2O2 stabilizes the monomeric enzyme form Rattus norvegicus
H2O2 stabilizes the monomeric enzyme form Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
DTT
-
 Homo sapiens
DTT
-
 Rattus norvegicus
JA-2 specific inhibition Homo sapiens
JA-2 specific inhibition Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
cytosol
-
 Homo sapiens 5829
-
mitochondrion
-
 Rattus norvegicus 5739
-
mitoplast
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc metalloproteinase Rattus norvegicus
Zn2+ a zinc metalloproteinase Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P52888
-
-
Rattus norvegicus P24155
-
-

Oxidation Stability

Oxidation Stability Organism
H2O2 activates the enzyme Rattus norvegicus
H2O2 activates the enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GFSPFRQ-EDDnp + H2O
-
 Rattus norvegicus ?
-
 ?
Abz-GFSPFRQ-EDDnp + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Rattus norvegicus
dimer
-
 Homo sapiens
monomer active form, stabilized by H2O2 Rattus norvegicus
monomer active form, stabilized by H2O2 Homo sapiens

Synonyms

Synonyms Comment Organism
THOP1
-
 Rattus norvegicus
THOP1
-
 Homo sapiens
TOP
-
 Rattus norvegicus
TOP
-
 Homo sapiens

General Information

General Information Comment Organism
additional information redox modulation of thimet oligopeptidase activity by hydrogen peroxide Rattus norvegicus
additional information redox modulation of thimet oligopeptidase activity by hydrogen peroxide Homo sapiens
physiological function thimet oligopeptidase (TOP) can process a diversity of bioactive peptides. TOP is one of the main postproteasomal enzymes that process peptide antigens in the MHC class I presentation route. Fine regulation of TOP activity by hydrogen peroxide Homo sapiens
physiological function thimet oligopeptidase (TOP) can process a diversity of bioactive peptides. TOP is one of the main postproteasomal enzymes that process peptide antigens in the MHC class I presentation route. Fine regulation of TOP activity by hydrogen peroxide. Contrary to the oxidation produced by aging associated with enzyme oligomerization and inhibition, H2O2 oxidation produces sulfenic acid and maintains recombinant TOP in the monomeric form. Consistent with the involvement of rTOP in a signaling redox cascade, the H2O2-oxidized recombinant TOP reacts with dimeric thioredoxin-1 (TRx-1) and remains covalently bound to one subunit of TRx-1 Rattus norvegicus