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Literature summary for 3.4.24.15 extracted from

  • Ferreira, J.C.; Icimoto, M.Y.; Marcondes, M.F.; Oliveira, V.; Nascimento, O.R.; Nantes, I.L.
    Recycling of the high valence States of heme proteins by cysteine residues of THIMET-oligopeptidase (2013), PLoS ONE, 8, e79102.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.15

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of GST-tagged enzyme in Escherichia coli Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme shows a broad tissue and subcellular compartment distribution Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc metalloendopeptidase Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
angiotensin I + H2O Homo sapiens
-
 ?
-
 ?
bradykinin + H2O Homo sapiens
-
 ?
-
 ?
gonadotropin-releasing hormone + H2O Homo sapiens
-
 ?
-
 ?
additional information Homo sapiens the enzyme is able to act as a reducing agent in the peroxidase cycle of myoglobin and horseradish peroxidase. The enzyme also prevents the formation of tryptophanyl radical in myoglobin challenged by H2O2 ?
-
 ?
neurotensin + H2O Homo sapiens
-
 ?
-
 ?
somatostatin + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P52888
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography to over 95% homogeneity, the protein is released from the GST fusion by cleavage with thrombin Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme shows a broad tissue and subcellular compartment distribution Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
 Homo sapiens ?
-
 ?
bradykinin + H2O
-
 Homo sapiens ?
-
 ?
gonadotropin-releasing hormone + H2O
-
 Homo sapiens ?
-
 ?
additional information the enzyme is able to act as a reducing agent in the peroxidase cycle of myoglobin and horseradish peroxidase. The enzyme also prevents the formation of tryptophanyl radical in myoglobin challenged by H2O2 Homo sapiens ?
-
 ?
neurotensin + H2O
-
 Homo sapiens ?
-
 ?
somatostatin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
thimet-oligopeptidase
-
 Homo sapiens
TOP
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
additional information H2O2 mechanism of enzyme oxidation by hydrogen peroxide, and proposed mechanism of TOP participation in the redox cycling of myoglobin, overview Homo sapiens
physiological function the thiol-rich enzyme enzyme is able to interact with the structure of myoglobin and horseradish peroxidase and acts as a reducing agent for the H2O2-promoted oxidized forms of these heme proteins Homo sapiens