Cloned (Comment) | Organism |
---|---|
expressed in HeLa cells and in Escherichia coli | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ELFSYLIEKVKR + H2O | purified TOP efficiently cleaves the nardilysin-dependent 12- and 13-residue precursors PRA(190-201) (ELFSYLIEKVKR) and PRA(190-202) (ELFSYLIEKVKRK) directly after the epitope's C-terminal Lys198 | Homo sapiens | ELFSYLIEK + VKR | - |
? | |
ELFSYLIEKVKRK + H2O | purified TOP efficiently cleaves the nardilysin-dependent 12- and 13-residue precursors PRA(190-201) (ELFSYLIEKVKR) and PRA(190-202) (ELFSYLIEKVKRK) directly after the epitopes C-terminal Lys198 | Homo sapiens | ELFSYLIEK + VKRK | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thimet oligopeptidase | - |
Homo sapiens |
TOP | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | suppression of TOP by RNAi results in much less recognition of K562-A3 cells by anti-ELFSYLIEK CTL indicating an involvement of TOP in the endogenous generation of ELFYSLIEK (CTL epitope) | Homo sapiens |
physiological function | Nardilysin and TOP are required, either together or alone, for the generation of a tumor-specific CTL epitope from PRAME, an immunodominant CTL epitope from Epstein-Barr virus protein EBNA3C, and a clinically important epitope from the melanoma protein MART-1. TOP functions as C-terminal trimming peptidase in antigen processing, and nardilysin contributes to both the C-terminal and N-terminal generation of CTL epitopes | Homo sapiens |