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Literature summary for 3.4.24.15 extracted from

  • Sugihara, Y.; Kawasaki, A.; Tsujimoto, Y.; Matsui, H.; Watanabe, K.
    Potencies of phosphine peptide inhibitors of mammalian thimet oligopeptidase and neurolysin on two bacterial pz peptidases (2007), Biosci. Biotechnol. Biochem., 71, 594-597.
    View publication on PubMed
Search for more literature for 3.4.24.15

Cloned(Commentary)

Cloned (Comment) Organism
Pz peptidases A and B overexpression in Escherichia coli strain BL21(DE3) Geobacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle specific for neurolysin Geobacillus sp.
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser specific for thimet oligopeptidase Geobacillus sp.
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr specific for thimet oligopeptidase Geobacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Collagen + H2O Geobacillus sp. degradation ?
-
 ?
Collagen + H2O Geobacillus sp. MO-1 degradation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus sp.
-
-
-
Geobacillus sp. MO-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Pz peptidases A and B from Escherichia coli strain BL21(DE3) by heat denaturation, anion exchange chromatography and gel filtration, 2.2fold and 8.4fold, respectively Geobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.5
-
 purified recombinant Pz peptidase B Geobacillus sp.
30.4
-
 purified recombinant Pz peptidase A Geobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O
-
 Geobacillus sp. ?
-
 ?
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O
-
 Geobacillus sp. MO-1 ?
-
 ?
Collagen + H2O degradation Geobacillus sp. ?
-
 ?
Collagen + H2O the enzyme recognizes the specific sequence Gly-Pro-Xaa of in collagen Geobacillus sp. ?
-
 ?
Collagen + H2O degradation Geobacillus sp. MO-1 ?
-
 ?
Collagen + H2O the enzyme recognizes the specific sequence Gly-Pro-Xaa of in collagen Geobacillus sp. MO-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
endopeptidase 24-15
-
 Geobacillus sp.
Pz peptidase A
-
 Geobacillus sp.
Pz peptidase B
-
 Geobacillus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000192
-
 Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle recombinant Pz peptidase B Geobacillus sp.
0.0000378
-
 Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser recombinant Pz peptidase B Geobacillus sp.
0.000043
-
 Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr recombinant Pz peptidase B Geobacillus sp.
0.0000877
-
 Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr recombinant Pz peptidase A Geobacillus sp.
0.0000889
-
 Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle recombinant Pz peptidase A Geobacillus sp.
0.0000901
-
 Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser recombinant Pz peptidase A Geobacillus sp.