Literature summary for 3.4.24.15 extracted from

Kawasaki, A.; Nakano, H.; Tsujimoto, Y.; Matsui, H.; Shimizu, T.; Nakatsu, T.; Kato, H.; Watanabe, K.
Crystallization and preliminary X-ray crystallographic studies of Pz peptidase A from Geobacillus collagenovorans MO-1 (2007), Acta Crystallogr. Sect. F, 63, 142-144.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of Pz peptidase A in Escherichia coli strain BL21 (DE3)	Geobacillus sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
Pz peptidase A with bound phosphine peptide inhibitor, 20 mg/ml protein in 50 mM Tris-HCl, pH 7.5, is incubated with 0.5 mM inhibitor for 24 h at 4 °C for complex formation, followed by hanging-drop vapour-diffusion method at 20°C for crystallization, mixing of 0.001 ml of protein and reservoir solution, the latter containing 12% w/v PEG 4000, 0.5 M magnesium acetate and 0.1 M Tris-HCl, pH 7.0, 5 days, cryoprotection in 14% w/v PEG 4000, 0.5 M magnesium acetate, 0.1 M TrisHCl, pH 7.0, and 10% v/v 2-propanol, X-ray diffraction structure determination and analysis at 1.80-1.90 A resolution	Geobacillus sp.

Crystallization (Comment)

Organism

Pz peptidase A with bound phosphine peptide inhibitor, 20 mg/ml protein in 50 mM Tris-HCl, pH 7.5, is incubated with 0.5 mM inhibitor for 24 h at 4 °C for complex formation, followed by hanging-drop vapour-diffusion method at 20°C for crystallization, mixing of 0.001 ml of protein and reservoir solution, the latter containing 12% w/v PEG 4000, 0.5 M magnesium acetate and 0.1 M Tris-HCl, pH 7.0, 5 days, cryoprotection in 14% w/v PEG 4000, 0.5 M magnesium acetate, 0.1 M TrisHCl, pH 7.0, and 10% v/v 2-propanol, X-ray diffraction structure determination and analysis at 1.80-1.90 A resolution

Geobacillus sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
benzyloxycarbonyl-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser	-	Geobacillus sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Geobacillus sp.	5622	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	Pz peptidase A is an intracellular M3 metallopeptidase	Geobacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Collagen + H2O	Geobacillus sp.	degradation by Pz peptidase A	?	-	?
Collagen + H2O	Geobacillus sp. MO-1	degradation by Pz peptidase A	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Geobacillus sp.	-	-	-
Geobacillus sp. MO-1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant Pz peptidase A from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography	Geobacillus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg + H2O	-	Geobacillus sp.	?	-	?
4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg + H2O	-	Geobacillus sp. MO-1	?	-	?
Collagen + H2O	degradation by Pz peptidase A	Geobacillus sp.	?	-	?
Collagen + H2O	Pz peptidase A recognizes collagen-specific tripeptide units, Gly-Pro-Xaa, Pz peptidase A shares common reactions with mammalian thimet oligopeptidase and neurolysin, but has extremely low primary sequence identity to these enzymes	Geobacillus sp.	?	-	?
Collagen + H2O	degradation by Pz peptidase A	Geobacillus sp. MO-1	?	-	?
Collagen + H2O	Pz peptidase A recognizes collagen-specific tripeptide units, Gly-Pro-Xaa, Pz peptidase A shares common reactions with mammalian thimet oligopeptidase and neurolysin, but has extremely low primary sequence identity to these enzymes	Geobacillus sp. MO-1	?	-	?

Synonyms

Synonyms	Comment	Organism
More	the enzyme belongs to the M3 peptidase family	Geobacillus sp.
Pz peptidase A	-	Geobacillus sp.
TOP	-	Geobacillus sp.