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Literature summary for 3.4.24.15 extracted from

  • Sigman, J.A.; Edwards, S.R.; Pabon, A.; Glucksman, M.J.; Wolfson, A.J.
    pH dependence studies provide insight into the structure and mechanism of thimet oligopeptidase (EC 3.4.24.15) (2003), FEBS Lett., 545, 224-228.
    View publication on PubMed
Search for more literature for 3.4.24.15

Protein Variants

Protein Variants Comment Organism
Y612F the ratio of turnover-number to Km-value is 0.2% of the wild-type ratio. The Ki-value for N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate is 2fold higher than the wild-type value Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl) pH 7.0, with Ca2+ Rattus norvegicus
0.0046
-
 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl) pH 7.8, wild-type enzyme Rattus norvegicus
0.0051
-
 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl) pH 7.8, mutant enzyme Y612F Rattus norvegicus
0.01
-
 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl) pH 7.0, without Ca2+ or at pH 5.8 with or without Ca2+ Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ increases the affinity for the substrate 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitropheyol Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P24155
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl) + H2O
-
 Rattus norvegicus ?
-
 ?
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl + H2O
-
 Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
TOP
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.027
-
 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl pH 7.8, mutant enzyme Y612F Rattus norvegicus
9.9
-
 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl pH 7.8, wild-type enzyme Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 45% of maximal activity, pH 9.5: about 40% of maximal activity Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000142
-
 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate pH 7.8, wild-type enzyme Rattus norvegicus
0.0000298
-
 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate pH 7.8, mutant enzyme Y612F Rattus norvegicus