Literature summary for 3.4.24.15 extracted from

Sigman, J.A.; Edwards, S.R.; Pabon, A.; Glucksman, M.J.; Wolfson, A.J.
pH dependence studies provide insight into the structure and mechanism of thimet oligopeptidase (EC 3.4.24.15) (2003), FEBS Lett., 545, 224-228.

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Protein Variants

Protein Variants	Comment	Organism
Y612F	the ratio of turnover-number to Km-value is 0.2% of the wild-type ratio. The Ki-value for N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate is 2fold higher than the wild-type value	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate	-	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.004	-	7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl)	pH 7.0, with Ca2+	Rattus norvegicus
0.0046	-	7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl)	pH 7.8, wild-type enzyme	Rattus norvegicus
0.0051	-	7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl)	pH 7.8, mutant enzyme Y612F	Rattus norvegicus
0.01	-	7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl)	pH 7.0, without Ca2+ or at pH 5.8 with or without Ca2+	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	increases the affinity for the substrate 7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitropheyol	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P24155	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-(2,4-dinitrophenyl) + H2O	-	Rattus norvegicus	?	-	?
7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl + H2O	-	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
TOP	-	Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.027	-	7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl	pH 7.8, mutant enzyme Y612F	Rattus norvegicus
9.9	-	7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenyl	pH 7.8, wild-type enzyme	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	8.5	-	Rattus norvegicus

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9.5	pH 7.0: about 45% of maximal activity, pH 9.5: about 40% of maximal activity	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000142	-	N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate	pH 7.8, wild-type enzyme	Rattus norvegicus
0.0000298	-	N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate	pH 7.8, mutant enzyme Y612F	Rattus norvegicus

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Release 2023.1 (January 2023)