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Literature summary for 3.4.24.15 extracted from
- Purification and characterization of Pz-peptidase B, a neutral metalloendopeptidase from bovine spermatozoa (1990), Biol. Reprod., 43, 643-658.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | reactivation by metal ions | Bos taurus |  |
| phosphoramidon | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90700 | - |
2 * 90700, SDS-PAGE | Bos taurus |
| 184000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
Pz peptidase A and B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spermatozoon | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C or -70°C, 3 months, more than 95% of the activity is retained | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg | - |
Bos taurus | 4-phenylazobenzyloxycarbonyl-Pro + Leu-Gly-Pro-D-Arg | - |
? | |
| aminobenzyl-Ala-Gly-Leu-Ala-nitrobenzylamide | weak | Bos taurus | aminobenzyl-Ala-Gly + Leu-Ala-nitrobenzylamide | - |
? | |
| Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + H2O | - |
Bos taurus | Asp-Arg-Val-Tyr + Ile-His-Pro-Phe | - |
? | |
| succinyl-Ala-Ala-Ala-4-nitroanilide | weak | Bos taurus | succinyl-Ala-Ala + Ala-4-nitroanilide | - |
? | |
| succinyl-Ala-Ala-Phe-4-nitroanilide | weak | Bos taurus | succinyl-Ala-Ala + Phe-4-nitroanilide | - |
? | |
| succinyl-Ala-Ala-Val-4-nitroanilide | weak | Bos taurus | succinyl-Ala-Ala + Val-4-nitroanilide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 90700, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | more than 50% of maximal activity at pH 6.5 and at pH 8.5 | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
irreversible inactivation at or below | Bos taurus |
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