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Literature summary for 3.4.24.15 extracted from
- Endopeptidase 24.15 from rat testes. Isolation of the enzyme and its specificity toward synthetic and natural peptides, including enkephalin-containing peptides (1989), Biochem. J., 261, 951-958.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Rattus norvegicus |  |
| EDTA | - |
Rattus norvegicus | |
| EGTA | - |
Rattus norvegicus | |
| leupeptin | weak | Rattus norvegicus | |
| NEM | - |
Rattus norvegicus | |
| PCMB | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu | - |
Rattus norvegicus | |
| 0.038 | - |
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro | - |
Rattus norvegicus | |
| 0.058 | - |
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
| 0.06 | - |
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile | - |
Rattus norvegicus | |
| 0.067 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
Rattus norvegicus | |
| 0.095 | - |
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 | - |
Rattus norvegicus | |
| 0.1 | - |
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
| 0.39 | - |
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
| 0.83 | - |
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
| 1.36 | - |
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
| 1.54 | - |
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate | - |
Rattus norvegicus | |
| 3.45 | - |
benzoyl-Gly-Ala-Ala-Gly-p-aminobenzoate | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | restores activity after EDTA treatment | Rattus norvegicus | |
| Zn2+ | restores activity after treatment with EDTA | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.3 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adrenorphin | - |
Rattus norvegicus | peptide fragments | - |
? | |
| Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | i.e. bradykinin, cleavage site: Phe5-Ser6 | Rattus norvegicus | Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg | - |
? | |
| benzoyl-Gly-Ala-Ala-Gly + H2O | - |
Rattus norvegicus | benzoyl-Gly + Ala-Ala-Gly | - |
? | |
| benzoyl-Gly-Ala-Ala-Gly-p-aminobenzoate | - |
Rattus norvegicus | benzoyl-Gly + Ala-Ala-Gly-p-aminobenzoate | - |
? | |
| benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate | - |
Rattus norvegicus | benzoyl-Gly + Ala-Ala-Leu-p-aminobenzoate | - |
? | |
| benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate | - |
? | |
| benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | benzoyl-Gly + Gly-Ala-Phe-p-aminobenzoate | - |
? | |
| benzoyl-Gly-Phe-Ala-Ala-Phe-p-aminobenzoate | inactive with benzoyl-Gly-D-Phe-Ala-Ala-Phe-p-aminobenzoate | Rattus norvegicus | benzoyl-Gly-Phe + Ala-Ala-Phe-p-aminobenzoate | - |
? | |
| benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | benzoyl-Gly + Phe-Ala-Phe-p-aminobenzoate | - |
? | |
| Met-enkephalin-Arg6-Gly7-Leu8 | - |
Rattus norvegicus | ? | - |
? | |
| additional information | cleaves preferentially bonds on the carboxyl side of hydrophobic amino acids | Rattus norvegicus | ? | - |
? | |
| pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 + H2O | i.e. luteinizing hormone-releasing hormone | Rattus norvegicus | pGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly-NH2 | - |
? | |
| pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu | i.e. neurotensin | Rattus norvegicus | pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu | - |
? | |
| tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | tert-butoxycarbonyl-Phe + Ala-Ala-Phe-p-aminobenzoate | - |
? | |
| Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O | i.e. dynorphin A(1-8) | Rattus norvegicus | Tyr-Gly-Gly-Phe-Leu + Arg-Arg-Ile | - |
? | |
| Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro | beta-neoendorphin | Rattus norvegicus | Tyr-Gly-Gly-Phe-Leu + Arg-Lys-Tyr-Pro | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.54 | - |
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
| 5.3 | - |
benzoyl-Gly-Ala-Ala-Gly | - |
Rattus norvegicus | |
| 7.9 | - |
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu | - |
Rattus norvegicus | |
| 12 | - |
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 | - |
Rattus norvegicus | |
| 17 | - |
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate | - |
Rattus norvegicus | |
| 19.2 | - |
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
| 33.8 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
Rattus norvegicus | |
| 37.9 | - |
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro | - |
Rattus norvegicus | |
| 48.3 | - |
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
| 86.4 | - |
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile | - |
Rattus norvegicus | |
| 94.1 | - |
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
| 179 | - |
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate | - |
Rattus norvegicus | |
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