Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.15 extracted from

  • Barrett, A.J.; Brown, M.A.
    Chicken liver Pz-peptidase, a thiol-dependent metallo-endopeptidase (1990), Biochem. J., 271, 701-706.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.15

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates at low concentrations Gallus gallus
dithiothreitol activates at low concentrations Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Gallus gallus
5-cyano-2-nitrothiobenzoate 0.1 mM, 62% inhibition Gallus gallus
diethyl dicarbonate reversed by hydroxylamine Gallus gallus
EDTA
-
 Gallus gallus
Fe2+
-
 Gallus gallus
iodoacetate 1 mM, 22% inhibition Gallus gallus
N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate
-
 Gallus gallus
NEM 1 mM, 82% inhibition Gallus gallus
Ni2+
-
 Gallus gallus
PCMB 0.1 mM, 100% inhibition Gallus gallus
tosyl-Phe-CH3Cl 0.1 mM, 15% inactivation Gallus gallus
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys i.e. dynorphin-(1-13) Gallus gallus
Zn2+ at high concentration Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0123
-
 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-(N6-(2,4-dinitrophenyl))Lys
-
 Gallus gallus
0.0137
-
 2,4-dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ restores activity after EDTA treatment Gallus gallus
Cd2+ restores activity after EDTA treatment Gallus gallus
Co2+ restores activity after EDTA treatment Gallus gallus
Mn2+ restores activity after EDTA treatment Gallus gallus
Zn2+ most effective ion in restoration of the activity after EDTA treatment Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Gallus gallus ?
-
 ?
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-(2,4-ditritrophenyl)Lys
-
 Gallus gallus ?
-
 ?
7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-(N6-(2,4-dinitrophenyl))Lys + H2O
-
 Gallus gallus ?
-
 ?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O i.e. bradykinin, cleavage site: Phe5-Ser6 Gallus gallus Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
-
 ?
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
 Gallus gallus benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate
-
 ?
additional information no hydrolysis of proteins Gallus gallus ?
-
 ?
N-acetyl-Ala-Ala-Ala
-
 Gallus gallus ?
-
 ?
N-acetyl-Ala-Ala-Ala-Ala
-
 Gallus gallus N-acetyl-Ala + Ala-Ala-Ala
-
 ?
phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg
-
 Gallus gallus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Gallus gallus