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Literature summary for 3.4.24.14 extracted from

  • Colige, A.; Ruggiero, F.; Vandenberghe, I.; Dubail, J.; Kesteloot, F.; Van Beeumen, J.; Beschin, A.; Brys, L.; Lapiere, C.M.; Nusgens, B.
    Domains and maturation processes that regulate the activity of ADAMTS-2, a metalloproteinase cleaving the aminopropeptide of fibrillar procollagens types I-III and V (2005), J. Biol. Chem., 280, 34397-34408.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
subcloned in a pCDNA3 vector, expression in HT1080, W126, COS, Balb, MCF7, SaOS2 and 293EBNA cells Bos taurus

Protein Variants

Protein Variants Comment Organism
additional information removal of the PNP domain, which significantly increases enzyme activity, removal of the fourth and the second TSPI repeats, which represses the enzymatic activity, no activity when lacking the central domain or mutated at the catalytic site, mutation of the potential cleavage site by furin strongly represses, but not abolishes enzyme activity, removal of adjacent domains completely suppresses activity, use of chimeric enzyme shows significant level of activity only when the metalloprotease domain or the other central domains are present, replacement of the C-terminal domains by GON-1 or ADAMTS-14 results in reduced activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
alpha2-Macroglobulin
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
177000
-
immunoprecipitation, full size enzyme after removal of the signal peptide Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
procollagen I + H2O
-
Bos taurus pCCollagen I + oligopeptide
-
?
procollagen V + H2O processes the aminopropetide at the end of the variable domain, cleavage sequence is different from previously described sites for ADAMTS-2 Bos taurus pCCollagen V + oligopeptide
-
?

Synonyms

Synonyms Comment Organism
ADAMTS-2
-
Bos taurus