Cloned (Comment) | Organism |
---|---|
subcloned in a pCDNA3 vector, expression in HT1080, W126, COS, Balb, MCF7, SaOS2 and 293EBNA cells | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | removal of the PNP domain, which significantly increases enzyme activity, removal of the fourth and the second TSPI repeats, which represses the enzymatic activity, no activity when lacking the central domain or mutated at the catalytic site, mutation of the potential cleavage site by furin strongly represses, but not abolishes enzyme activity, removal of adjacent domains completely suppresses activity, use of chimeric enzyme shows significant level of activity only when the metalloprotease domain or the other central domains are present, replacement of the C-terminal domains by GON-1 or ADAMTS-14 results in reduced activity | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha2-Macroglobulin | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
177000 | - |
immunoprecipitation, full size enzyme after removal of the signal peptide | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen I + H2O | - |
Bos taurus | pCCollagen I + oligopeptide | - |
? | |
procollagen V + H2O | processes the aminopropetide at the end of the variable domain, cleavage sequence is different from previously described sites for ADAMTS-2 | Bos taurus | pCCollagen V + oligopeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADAMTS-2 | - |
Bos taurus |