Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Neisseria gonorrhoeae | |
bathocuproine disulfonic acid | - |
Neisseria gonorrhoeae | |
EDTA | - |
Neisseria gonorrhoeae | |
octyl glucoside | - |
Neisseria gonorrhoeae | |
phenylmethylsulfonyl fluoride | inhibition of the type 1 protease, no inhibition of the type 2 protease | Neisseria gonorrhoeae | |
Sarcosyl | - |
Neisseria gonorrhoeae | |
SDS | - |
Neisseria gonorrhoeae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
112000 | - |
x * 112000, type 1 protease, SDS-PAGE | Neisseria gonorrhoeae |
114000 | - |
x * 114000, the 114000 Da peptide is converted to a still active 109000 Da peptide during isolation, type 2 protease, SDS-PAGE | Neisseria gonorrhoeae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria gonorrhoeae | - |
type 1 and type 2 enzyme | - |
Purification (Comment) | Organism |
---|---|
type 1 enzyme | Neisseria gonorrhoeae |
type 2 enzyme | Neisseria gonorrhoeae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | the enzyme is secreted into the culture medium throughout the exponential growth | Neisseria gonorrhoeae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Neisseria gonorrhoeae |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-acetate, pH 7.5, 20% glycerol, 0.1 mM dithiothreitol, 5 mM EDTA, type 1 protease, less than 10% loss of activity after 2 weeks | Neisseria gonorrhoeae |
4°C, long term storage of the 109000 Da type 2 protease, breakdown yields fragments of 95000 and 15000 Da and loss of activity | Neisseria gonorrhoeae |
50 mM, Tris-HCl, pH 7.5, 20% glycerol, stable for at least 2 months | Neisseria gonorrhoeae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
immunoglobulin A1 + H2O | alpha-chain | Neisseria gonorrhoeae | oligopeptides derived from immunoglobulin A1 | - |
? | |
immunoglobulin A1 + H2O | type 2 enzyme cleaves peptide bond 235-236, type 1 enzyme cleaves bond 237-238 in the alpha chain of IgA | Neisseria gonorrhoeae | oligopeptides derived from immunoglobulin A1 | - |
? | |
immunoglobulin A1 + H2O | the activity level of the type 2 enzyme is 10fold that observed for the type 1 enzyme | Neisseria gonorrhoeae | oligopeptides derived from immunoglobulin A1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 112000, type 1 protease, SDS-PAGE | Neisseria gonorrhoeae |
? | x * 114000, the 114000 Da peptide is converted to a still active 109000 Da peptide during isolation, type 2 protease, SDS-PAGE | Neisseria gonorrhoeae |