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Literature summary for 3.4.24.11 extracted from
- Purification and specificity of a membrane-bound metalloendopeptidase from bovine pituitaries (1981), Biochemistry, 20, 4942-4950.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Bos taurus |  |
| 2-mercaptoethanol | - |
Bos taurus | |
| diisopropyl fluorophosphate | - |
Bos taurus | |
| dithiothreitol | - |
Bos taurus | |
| EDTA | - |
Bos taurus | |
| GSH | - |
Bos taurus | |
| L-Cys | - |
Bos taurus | |
| pepstatin | - |
Bos taurus | |
| phosphate | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 0.08 | - |
Nalpha-benzoyl-Gly-Lys-Arg-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 0.11 | - |
Nalpha-benzoyl-Gly-Arg-Arg-Phe-2-naphthylamide | - |
Bos taurus | |
| 0.11 | - |
Nalpha-benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 0.13 | - |
N-benzyoxycarbonyl-Gly-Gly-Leu 2-naphthylamide | - |
Bos taurus | |
| 0.18 | - |
Nalpha-benzoyl-Gly-Arg-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 0.19 | - |
Nalpha-benzoyl-Gly-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 0.3 | - |
Nalpha-benzoyl-Gly-Arg-Arg-Ala-2-naphthylamide | - |
Bos taurus | |
| 0.42 | - |
N-(4-carboxybutanoyl)-L-alanyl-L-alanyl-N-(4-methoxynaphthalen-2-yl)-L-phenylalaninamide | - |
Bos taurus | |
| 0.45 | - |
succinyl-Arg-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 0.59 | - |
glutaryl-Ala-Ala-Phe-2-naphthylamide | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Bos taurus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hypophysis | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide + H2O | - |
Bos taurus | benzoyl-Gly-Gly-Arg + L-Leu-2-naphthylamide | - |
? | |
| glutaryl-Ala-Ala-Phe-2-naphthylamide + H2O | - |
Bos taurus | glutaryl-Ala-Ala + Phe-2-naphthylamide | - |
? | |
| glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamide + H2O | - |
Bos taurus | glutaryl-Ala-Ala + Phe-4-methoxy-2-naphthylamide | - |
? | |
| hippuryl-Arg-Arg-Leu-2-naphthylamide + H2O | - |
Bos taurus | hippuryl-Arg-Arg + Leu-2-naphthylamide | - |
? | |
| N-benzyloxycarbonyl-Gly-Gly-Leu 2-naphthylamide + H2O | - |
Bos taurus | N-benzyloxycarbonyl-Gly-Gly + L-leucine 2-naphthylamide | - |
? | |
| N-benzyoxycarbonyl-Gly-Gly-Leu 2-naphthylamide + H2O | - |
Bos taurus | ? | - |
? | |
| N-benzyoxycarbonyl-Gly-Gly-Leu-2-naphthylamide + H2O | - |
Bos taurus | N-benzyoxycarbonyl-Gly-Gly + L-Leu-2-naphthylamide | - |
? | |
| Nalpha-benzoyl-Gly-Arg-Arg-Ala-2-naphthylamide + H2O | - |
Bos taurus | Nalpha-benzoyl-Gly-Arg-Arg + Ala-2-naphthylamide | - |
? | |
| Nalpha-benzoyl-Gly-Arg-Arg-Leu-2-naphthylamide + H2O | - |
Bos taurus | Nalpha-benzoyl-Gly-Arg-Arg + Leu-2-naphthylamide | - |
? | |
| Nalpha-benzoyl-Gly-Arg-Arg-Phe-2-naphthylamide + H2O | - |
Bos taurus | Nalpha-benzoyl-Gly-Arg-Arg + Phe-2-naphthylamide | - |
? | |
| Nalpha-benzoyl-Gly-Arg-Leu-2-naphthylamide + H2O | - |
Bos taurus | Nalpha-benzoyl-Gly-Arg + Leu-2-naphthylamide | - |
? | |
| Nalpha-benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide + H2O | - |
Bos taurus | Nalpha-benzoyl-Gly-Gly-Arg + Leu-2-naphthylamide | - |
? | |
| Nalpha-benzoyl-Gly-Lys-Arg-Arg-Leu-2-naphthylamide + H2O | - |
Bos taurus | Nalpha-benzoyl-Gly-Lys-Arg-Arg + Leu-2-naphthylamide | - |
? | |
| Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg-Leu-2-naphthylamide + H2O | - |
Bos taurus | Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg + Leu-2-naphthylamide | - |
? | |
| succinyl-Arg-Arg-Leu-2-naphthylamide + H2O | - |
Bos taurus | succinyl-Arg-Arg + Leu-2-naphthylamide | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0483 | - |
Nalpha-benzoyl-Gly-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 0.05 | - |
benzoyl-Gly-Gly-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 0.105 | - |
N-benzyoxycarbonyl-Gly-Gly-Leu-2-naphthylamide | - |
Bos taurus | |
| 0.147 | - |
Nalpha-benzoyl-Gly-Arg-Arg-Ala-2-naphthylamide | - |
Bos taurus | |
| 0.383 | - |
Nalpha-benzoyl-Gly-Lys-Lys-Arg-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 1.82 | - |
Nalpha-benzoyl-Gly-Lys-Arg-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 2 | - |
Nalpha-benzoyl-Gly-Arg-Arg-Phe-2-naphthylamide | - |
Bos taurus | |
| 4 | - |
Nalpha-benzoyl-Gly-Arg-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 5.28 | - |
glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamide | - |
Bos taurus | |
| 6.32 | - |
succinyl-Arg-Arg-Leu-2-naphthylamide | - |
Bos taurus | |
| 9.87 | - |
glutaryl-Ala-Ala-Phe-2-naphthylamide | - |
Bos taurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.8 | hydrolysis of hippuryl-Arg-Arg-Leu-2-naphthylamide | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.4 | about 80% of maximal activity at pH 7.0 and at pH 8.4, hydrolysis of hippuryl-Arg-Arg-Leu-2-naphthylamide | Bos taurus |
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