Cloned (Comment) | Organism |
---|---|
cloned into the pQE expression vector, resulting in the fusion of a hexahistidine at the N-terminus, expression in Escherichia coli | Lactococcus lactis |
Protein Variants | Comment | Organism |
---|---|---|
E535Q | inactive enzyme | Lactococcus lactis |
H587Q | inactive enzyme | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | - |
Lactococcus lactis | |
phosphoramidon | - |
Lactococcus lactis | |
thiorphan | weak | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
angiotensin I | - |
Lactococcus lactis | |
0.0018 | - |
Vasoactive intestinal peptide | - |
Lactococcus lactis | |
0.0028 | - |
adrenocorticotropic hormone | - |
Lactococcus lactis | |
0.005 | - |
bradykinin | - |
Lactococcus lactis | |
0.00654 | - |
beta-endorphin | - |
Lactococcus lactis | |
0.0082 | - |
dynorphin A-17 | - |
Lactococcus lactis | |
0.01 | - |
Substance P | - |
Lactococcus lactis | |
0.01 | - |
dynorphin A-13 | - |
Lactococcus lactis | |
0.025 | - |
dynorphin A-6 | - |
Lactococcus lactis | |
0.035 | - |
dynorphin A-10 | - |
Lactococcus lactis | |
0.04 | - |
dynorphin A-9 | - |
Lactococcus lactis | |
0.1 | - |
Met-enkephalin | - |
Lactococcus lactis | |
0.135 | - |
alpha-neoendorphin | - |
Lactococcus lactis | |
0.2 | - |
Leu-enkephalin | - |
Lactococcus lactis | |
0.298 | - |
dynorphin A-8 | - |
Lactococcus lactis | |
0.41 | - |
Met-enkephalin amide | - |
Lactococcus lactis | |
0.612 | - |
Leu-enkephalinamide | - |
Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.203 | - |
cleavage of succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adrenocorticotropic hormone + H2O | - |
Lactococcus lactis | ? | - |
? | |
alpha-neoendorphin + H2O | - |
Lactococcus lactis | ? | - |
? | |
angiotensin I + H2O | - |
Lactococcus lactis | ? | - |
? | |
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O | - |
Lactococcus lactis | ? | - |
? | |
beta-endorphin + H2O | - |
Lactococcus lactis | ? | - |
? | |
beta-neoendorphin + H2O | - |
Lactococcus lactis | ? | - |
? | |
bradykinin + H2O | - |
Lactococcus lactis | ? | - |
? | |
dynorphin + H2O | dynorphin A-6, A-8, A-9, A-10, A-13 and A-17 | Lactococcus lactis | ? | - |
? | |
dynorphin A-10 + H2O | - |
Lactococcus lactis | ? | - |
? | |
dynorphin A-13 + H2O | - |
Lactococcus lactis | ? | - |
? | |
dynorphin A-17 + H2O | - |
Lactococcus lactis | ? | - |
? | |
dynorphin A-6 + H2O | - |
Lactococcus lactis | ? | - |
? | |
dynorphin A-8 + H2O | - |
Lactococcus lactis | ? | - |
? | |
dynorphin A-9 + H2O | - |
Lactococcus lactis | ? | - |
? | |
enkephalin + H2O | - |
Lactococcus lactis | ? | - |
? | |
Leu-enkephalin + H2O | - |
Lactococcus lactis | ? | - |
? | |
Leu5-enkephalinamide + H2O | - |
Lactococcus lactis | ? | - |
? | |
Met-enkephalin + H2O | - |
Lactococcus lactis | ? | - |
? | |
Met-enkephalin amide + H2O | - |
Lactococcus lactis | ? | - |
? | |
Substance P + H2O | - |
Lactococcus lactis | ? | - |
? | |
succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Lactococcus lactis | ? | - |
? | |
Vasoactive intestinal peptide + H2O | - |
Lactococcus lactis | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
Met-enkephalin amide | - |
Lactococcus lactis | |
1.7 | - |
adrenocorticotropic hormone | - |
Lactococcus lactis | |
1.8 | - |
Vasoactive intestinal peptide | - |
Lactococcus lactis | |
4.3 | - |
bradykinin | - |
Lactococcus lactis | |
7.8 | - |
Met-enkephalin | - |
Lactococcus lactis | |
8.2 | - |
Leu-enkephalinamide | - |
Lactococcus lactis | |
14 | - |
dynorphin A-6 | - |
Lactococcus lactis | |
15 | - |
Leu-enkephalin | - |
Lactococcus lactis | |
18.6 | - |
dynorphin A-13 | - |
Lactococcus lactis | |
19.6 | - |
dynorphin A-8 | - |
Lactococcus lactis | |
23.8 | - |
dynorphin A-10 | - |
Lactococcus lactis | |
25.4 | - |
dynorphin A-17 | - |
Lactococcus lactis | |
26 | - |
beta-endorphin | - |
Lactococcus lactis | |
28 | - |
Substance P | - |
Lactococcus lactis | |
32.2 | - |
dynorphin A-9 | - |
Lactococcus lactis | |
44.6 | - |
alpha-neoendorphin | - |
Lactococcus lactis |