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Literature summary for 3.4.24.11 extracted from

  • Fulcher, I.S.; Kenny, A.J.
    Proteins of the kidney microvillar membrane. The amphipathic forms of endopeptidase purified from pig kidneys (1983), Biochem. J., 211, 743-753.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Sus scrofa
2,3-Dimercaptopropan-1-ol weak Sus scrofa
dithiothreitol weak Sus scrofa
EDTA
-
Sus scrofa
phosphoramidon
-
Sus scrofa
puromycin
-
Sus scrofa
thiorphan
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0685
-
glutaryl-Gly-Gly-Phe-2-naphthylamide
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc kidney enzyme contains 1 Zn atom per subunit Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
x * 89000, kidney enzyme, SDS-PAGE Sus scrofa
320000
-
kidney enzyme, gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification kidney enzyme contains 15% carbohydrate, if glucose is excluded Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney cortex Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-Gly-Gly-Phe-2-naphthylamide + H2O
-
Sus scrofa glutaryl-Gly-Gly + Phe-2-naphthylamide
-
?

Subunits

Subunits Comment Organism
? x * 89000, kidney enzyme, SDS-PAGE Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
43.7
-
glutaryl-Gly-Gly-Phe-2-naphthylamide
-
Sus scrofa