Application | Comment | Organism |
---|---|---|
medicine | NEP is a target for Entresto used in treatment of heart failure | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition | Homo sapiens | |
additional information | structure-activity relationship studies. No or poor inhibition by 4-(2-aminoethyl)benzylsulfonyl fluoride (AEBSF), and Nalpha-tosyl-l-lysyl chloromethylketone (TLCK), and iodoacetamide | Homo sapiens | |
PMSF | weak inhibition | Homo sapiens | |
thiorphan | complete inhibition | Homo sapiens | |
tris(2-carboxyethyl)phosphine | TCEP, strong inhibition | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08473 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from human blood plasma by ammonium sulfate fractionation, and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Homo sapiens | - |
heart | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 KFRRQRPRLSHKGPMPF + 2 H2O | - |
Homo sapiens | KFRRQRPR + LSHKGPMPF + KFRRQRPRL + SHKGPMPF | - |
ir | |
2 LVQPRGSRNGPGPWQGGRRKFRRQRPRLSHKGPMPF + 2 H2O | - |
Homo sapiens | LVQPRGSRNGPGPWQGGRRKFRRQRPRL + SHKGPMPF + LVQPRGSRNGPGPWQGGRRKFRRQRPR + LSHKGPMPF | - |
ir | |
2 pGlu-RPRLSHKGPMPF + 2 H2O | - |
Homo sapiens | pGlu-RPRL + pGlu-RPR + SHKGPMPF + LSHKGPMPF | - |
ir | |
Abz-QRPRLSH-(3-nitro)Tyr + H2O | - |
Homo sapiens | Abz-QRPRL + Ser-His-(3-nitro)Tyr | - |
ir | |
additional information | in vitro NEP proteolysis generates fragments that lack the ability to bind to the apelin receptor, NEP fully inactivates apelin | Homo sapiens | ? | - |
- |
|
additional information | in vitro NEP proteolysis generates fragments that lack the ability to bind to the apelin receptor, NEP fully inactivates apelin | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NEP | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | isoelectric focusing | Homo sapiens |
6.5 | - |
enzyme assay | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | activity range, no activity at pH 5.0 | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the metalloprotease neprilysin degrades and inactivates apelin peptides. The apelinergic system is a mammalian peptide hormone network with key physiological roles. Apelin isoforms and analogues are believed to be promising therapeutics for cardiovascular disease. Involvement of NEP in the apelinergic system. In vitro NEP proteolysis generates fragments that lack the ability to bind to the apelin receptor, NEP protease fully inactivates apelin | Homo sapiens |