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Literature summary for 3.4.23.B8 extracted from

  • Li, C.; Li, X.; Lu, W.
    Total chemical synthesis of human T-cell leukemia virus type 1 protease via native chemical ligation (2010), Biopolymers, 94, 487-494.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.B8

Protein Variants

Protein Variants Comment Organism
additional information chemical synthesis of a full-length HTLV protease and a C-terminally truncated form encompassing residues 1-116. Constructs contain an S47C mutation for native chemical ligation and an L40I mutation that suppresses known autolysis of the protease without altering its catalytic activity. Truncation of the C-terminal tail lowers the turnover number of the viral enzyme by a factor of 2 and its catalytic efficiency by roughly tenfold Human T-cell leukemia virus type I

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 APQVLF(NO2)VMHPL full length enzyme, 37°C, pH 5.6 Human T-cell leukemia virus type I
0.062
-
 APQVLF(NO2)VMHPL truncated enzyme, residues 1-116, 37°C, pH 5.6 Human T-cell leukemia virus type I
0.38
-
 APQVLPVMHP truncated enzyme, residues 1-116, 37°C, pH 5.6 Human T-cell leukemia virus type I
0.52
-
 KTKVLVVQPK full length enzyme, 37°C, pH 5.6 Human T-cell leukemia virus type I
0.8
-
 APQVLPVMHP full length enzyme, 37°C, pH 5.6 Human T-cell leukemia virus type I
1.5 2 KTKVLVVQPK truncated enzyme, residues 1-116, 37°C, pH 5.6 Human T-cell leukemia virus type I

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13672
-
 x * 13672, ESI-MS of full length enzyme Human T-cell leukemia virus type I

Organism

Organism UniProt Comment Textmining
Human T-cell leukemia virus type I
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
APQVLF(NO2)VMHPL + H2O
-
 Human T-cell leukemia virus type I APQVL + F(NO2)VMHP
-
 ?
APQVLPVMHP + H2O
-
 Human T-cell leukemia virus type I APQVL + PVMHP
-
 ?
KTKVLVVQPK + H2O
-
 Human T-cell leukemia virus type I KTKVL + VVQPK
-
 ?

Subunits

Subunits Comment Organism
? x * 13672, ESI-MS of full length enzyme Human T-cell leukemia virus type I

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
 APQVLF(NO2)VMHPL truncated enzyme, residues 1-116, 37°C, pH 5.6 Human T-cell leukemia virus type I
0.161
-
 APQVLF(NO2)VMHPL full length enzyme, 37°C, pH 5.6 Human T-cell leukemia virus type I
2.8
-
 KTKVLVVQPK truncated enzyme, residues 1-116, 37°C, pH 5.6 Human T-cell leukemia virus type I
4.3
-
 APQVLPVMHP truncated enzyme, residues 1-116, 37°C, pH 5.6 Human T-cell leukemia virus type I
6.7
-
 KTKVLVVQPK full length enzyme, 37°C, pH 5.6 Human T-cell leukemia virus type I
8.3
-
 APQVLPVMHP full length enzyme, 37°C, pH 5.6 Human T-cell leukemia virus type I

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.37
-
 APQVLF(NO2)VMHPL truncated enzyme, residues 1-116, 37°C, pH 5.6 Human T-cell leukemia virus type I
1.14
-
 APQVLPVMHP truncated enzyme, residues 1-116, 37°C, pH 5.6 Human T-cell leukemia virus type I
1.87
-
 KTKVLVVQPK truncated enzyme, residues 1-116, 37°C, pH 5.6 Human T-cell leukemia virus type I
3.82
-
 APQVLF(NO2)VMHPL full length enzyme, 37°C, pH 5.6 Human T-cell leukemia virus type I
10.4
-
 APQVLPVMHP full length enzyme, 37°C, pH 5.6 Human T-cell leukemia virus type I
12.8
-
 KTKVLVVQPK full length enzyme, 37°C, pH 5.6 Human T-cell leukemia virus type I