Literature summary for 3.4.23.B8 extracted from

Satoh, T.; Li, M.; Nguyen, J.T.; Kiso, Y.; Gustchina, A.; Wlodawer, A.
Crystal structures of inhibitor complexes of human T-cell leukemia virus (HTLV-1) protease (2010), J. Mol. Biol., 401, 626-641.

Search for more literature for 3.4.23.B8

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta2(DE3)pLysS cells	Human T-cell leukemia virus type I

Crystallization (Commentary)

Crystallization (Comment)	Organism
HTLV-1 PR complexed with Ac-Ala-Pro-Gln-Val-Sta-Val-Met-His-Pro, hanging drop vapor diffusion method, using 17% (w/v) PEG 8000, 16% PEG (w/v) 300, 0.1 M sodium acetate (pH 5.2), at 20°C. HTLV-1 PR complexed with KNI-10562, hanging drop vapor diffusion method, using 26% (w/v) pentaerythritol ethoxylate and 0.2 M ZnSO4 in 0.1 M Bis-Tris buffer (pH 5.2), at 20°C. HTLV-1PR complexed with KNI-10683, hanging drop vapor diffusion method, using 17% (w/v) PEG 8000, 16% (w/v) PEG 300, 10 mM dithiothreitol, and 0.2M KH2PO4 in 0.1 M Bis-Tris buffer, pH 5.2, at 20°C	Human T-cell leukemia virus type I

Crystallization (Comment)

Organism

HTLV-1 PR complexed with Ac-Ala-Pro-Gln-Val-Sta-Val-Met-His-Pro, hanging drop vapor diffusion method, using 17% (w/v) PEG 8000, 16% PEG (w/v) 300, 0.1 M sodium acetate (pH 5.2), at 20°C. HTLV-1 PR complexed with KNI-10562, hanging drop vapor diffusion method, using 26% (w/v) pentaerythritol ethoxylate and 0.2 M ZnSO4 in 0.1 M Bis-Tris buffer (pH 5.2), at 20°C. HTLV-1PR complexed with KNI-10683, hanging drop vapor diffusion method, using 17% (w/v) PEG 8000, 16% (w/v) PEG 300, 10 mM dithiothreitol, and 0.2M KH2PO4 in 0.1 M Bis-Tris buffer, pH 5.2, at 20°C

Human T-cell leukemia virus type I

Protein Variants

Protein Variants	Comment	Organism
L40I	the mutation of the protease prevents autolysis and enhances enzyme stability and substrate cleavage	Human T-cell leukemia virus type I

Inhibitors

Inhibitors	Comment	Organism
Ac-Ala-Pro-Gln-Val-Sta-Val-Met-His-Pro	19% inhibition at 50 nM	Human T-cell leukemia virus type I
KNI-10220	HTLV-1 PR-specific inhibitor, 61% inhibition at 50 nM	Human T-cell leukemia virus type I
KNI-10296c	HTLV-1 PR-specific inhibitor, 28% inhibition at 50 nM	Human T-cell leukemia virus type I
KNI-10516	HTLV-1 PR-specific inhibitor, 86% inhibition at 50 nM	Human T-cell leukemia virus type I
KNI-10562	78% inhibition at 50 nM	Human T-cell leukemia virus type I
KNI-10673	29% inhibition at 50 nM	Human T-cell leukemia virus type I
KNI-10681	HTLV-1 PR-specific inhibitor, 40% inhibition at 50 nM	Human T-cell leukemia virus type I
KNI-10683	HTLV-1 PR-specific inhibitor, 76% inhibition at 50 nM	Human T-cell leukemia virus type I
KNI-10729	HTLV-1 PR-specific inhibitor, 79% inhibition at 50 nM	Human T-cell leukemia virus type I
KNI-1595b	HTLV-1 PR-specific inhibitor, 5% inhibition at 50 nM	Human T-cell leukemia virus type I

Organism

Organism	UniProt	Comment	Textmining
Human T-cell leukemia virus type I	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q Sepharose column chromatography and SP Sepharose column chromatography	Human T-cell leukemia virus type I

Renatured (Commentary)

Renatured (Comment)	Organism
the purified unfolded HTLV-1 PR is refolded by dialysis against 15 mM sodium acetate (pH 3.0), 5% (w/v) PEG 300, and 5 mM dithiothreitol	Human T-cell leukemia virus type I

Subunits

Subunits	Comment	Organism
trimer	the trimer consists of three homodimers	Human T-cell leukemia virus type I

Synonyms

Synonyms	Comment	Organism
HTLV-1 PR	-	Human T-cell leukemia virus type I