Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes as maltose-binding protein fusion proteins | Human T-cell leukemia virus type I |
Protein Variants | Comment | Organism |
---|---|---|
A59I | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no folding | Human T-cell leukemia virus type I |
F67Q | site-directed mutagenesis, inactive mutant, no folding | Human T-cell leukemia virus type I |
L57G | site-directed mutagenesis, inactive mutant, no folding | Human T-cell leukemia virus type I |
M37A | site-directed mutagenesis, inactive mutant, no folding | Human T-cell leukemia virus type I |
M37D | site-directed mutagenesis, inactive mutant, no folding | Human T-cell leukemia virus type I |
M37I | site-directed mutagenesis, reduced activity and highly reduced folding efficiency compared to the wild-type enzyme | Human T-cell leukemia virus type I |
M37N | site-directed mutagenesis, inactive mutant, no folding | Human T-cell leukemia virus type I |
M37V | site-directed mutagenesis, reduced activity and folding efficiency compared to the wild-type enzyme | Human T-cell leukemia virus type I |
N96T | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no folding | Human T-cell leukemia virus type I |
N96T/N97P/W98V | site-directed mutagenesis, inactive mutant, no folding | Human T-cell leukemia virus type I |
N97P | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no folding | Human T-cell leukemia virus type I |
V56I | site-directed mutagenesis, inactive mutant, no folding | Human T-cell leukemia virus type I |
V56I/L57G/A59I | site-directed mutagenesis, reduced activity and folding efficiency compared to the wild-type enzyme | Human T-cell leukemia virus type I |
V56I/L57G/A59I/N96T/N97P/W98V | site-directed mutagenesis, inactive mutant, no folding | Human T-cell leukemia virus type I |
W98V | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme, no folding | Human T-cell leukemia virus type I |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes | Human T-cell leukemia virus type I |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | activates at 4 M | Human T-cell leukemia virus type I |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human T-cell leukemia virus type I | - |
HTLV-I | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from inclusion bodies | Human T-cell leukemia virus type I |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from inclusion bodies by dialysis | Human T-cell leukemia virus type I |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Human T-cell leukemia virus type I |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
APQVLPVMHP + H2O | - |
Human T-cell leukemia virus type I | APQVL + PVMHP | - |
? | |
dabsyl-KTKVLVVQPK-EDANS + H2O | i.e. peptide substrate IB268, fluorescent-labeled | Human T-cell leukemia virus type I | dabsyl-KTKVL + VVQPK-EDANS | - |
? | |
DELILPVKRK + H2O | - |
Human T-cell leukemia virus type I | DELIL + PVKRK | - |
? | |
DKELYPLTSL + H2O | low activity | Human T-cell leukemia virus type I | DKELY + PLTSL | - |
? | |
DPASILPVIP + H2O | - |
Human T-cell leukemia virus type I | DPASIL + PVIP | - |
? | |
IPFAAAQQRK + H2O | - |
Human T-cell leukemia virus type I | IPFAA + AQQRK | - |
? | |
IQPLIMAVVNR + H2O | good substrate | Human T-cell leukemia virus type I | IQPLIM + AVVNR | - |
? | |
IRKILFLDG + H2O | - |
Human T-cell leukemia virus type I | IRKIL + FLDG | - |
? | |
IRQVLFLEKI + H2O | good substrate | Human T-cell leukemia virus type I | IRQVL + FLEKI | - |
? | |
KGPPVILPIQAP + H2O | - |
Human T-cell leukemia virus type I | KGPPVIL + PIQAP | - |
? | |
KTKVLVVQPK + H2O | i.e. peptide substrate IB268, good substrate | Human T-cell leukemia virus type I | KTKVL + VVQPK | - |
? | |
LTFTFPVVFMRR + H2O | low activity | Human T-cell leukemia virus type I | LTFTF + PVVFMRR | - |
? | |
additional information | specificity of wild-type and mutant HTLV-1 PRs with diverse cleavage sites, overview | Human T-cell leukemia virus type I | ? | - |
? | |
PKDIFPVTET + H2O | - |
Human T-cell leukemia virus type I | PKDIF + PVTET | - |
? | |
PLQVLTLNIERR + H2O | - |
Human T-cell leukemia virus type I | PLQVL + TLNIERR | - |
? | |
PPAILPIISE + H2O | good substrate | Human T-cell leukemia virus type I | PPAIL + PIISE | - |
? | |
PPMVGVLDAP + H2O | - |
Human T-cell leukemia virus type I | PPMVG + VLDAP | - |
? | |
PVVAMPVVIK + H2O | - |
Human T-cell leukemia virus type I | PVVAM + PVVIK | - |
? | |
RATVLTVALH + H2O | - |
Human T-cell leukemia virus type I | RATVL + TVALH | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular structure modeling of wild-type enzyme with bound substrate KTKVLVVQPK | Human T-cell leukemia virus type I |
Synonyms | Comment | Organism |
---|---|---|
HTLV-I PR | - |
Human T-cell leukemia virus type I |
HTLV-I protease | - |
Human T-cell leukemia virus type I |
human T-cell leukemia virus type 1 protease | - |
Human T-cell leukemia virus type I |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Human T-cell leukemia virus type I |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
assay at | Human T-cell leukemia virus type I |