Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzyme in Escherichia coli strain BL21(DE3) | Human T-cell leukemia virus type I |
Protein Variants | Comment | Organism |
---|---|---|
L40I | site-directed mutagenesis, modified wild-type protease | Human T-cell leukemia virus type I |
additional information | construction of a L40I mutant enzyme lacking the 10 C-terminal amino acid residues, the mutant shows slightly increased activity compared to the wild-type enzyme | Human T-cell leukemia virus type I |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
dabsyl-(GABA)-PQVLPVMH-EDANS | pH 5.2, 37°C, modified L40I mutant enzyme lacking the 10 C-terminal amino acid residues | Human T-cell leukemia virus type I | |
0.01 | - |
dabsyl-(GABA)-PQVLPVMH-EDANS | pH 5.2, 37°C, modified L40I wild-type enzyme | Human T-cell leukemia virus type I |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human T-cell leukemia virus type I | - |
HTLV-I | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-(GABA)-PQVLPVMH-EDANS + H2O | fluorogenic peptide substrate containing a MA/CA cleavage site | Human T-cell leukemia virus type I | dabsyl-(GABA)-PQVL + PVMH-EDANS | - |
? | |
additional information | the 10 C-terminal residues of HTLV-I protease are not necessary for enzymatic activity | Human T-cell leukemia virus type I | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the 10 C-terminal residues of HTLV-I protease are not necessary for enzymatic activity, structure model of wild-type enzyme and mutant enzyme lacking the 10 C-terminal amino acid residues | Human T-cell leukemia virus type I |
Synonyms | Comment | Organism |
---|---|---|
HTLV-I protease | - |
Human T-cell leukemia virus type I |
human T-lymphotropic virus type I protease | - |
Human T-cell leukemia virus type I |
More | belongs to the leukemic proteases | Human T-cell leukemia virus type I |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Human T-cell leukemia virus type I |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0046 | - |
dabsyl-(GABA)-PQVLPVMH-EDANS | pH 5.2, 37°C, modified L40I mutant enzyme lacking the 10 C-terminal amino acid residues | Human T-cell leukemia virus type I | |
0.0057 | - |
dabsyl-(GABA)-PQVLPVMH-EDANS | pH 5.2, 37°C, modified L40I wild-type enzyme | Human T-cell leukemia virus type I |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
assay at | Human T-cell leukemia virus type I |