Literature summary for 3.4.23.B8 extracted from

Saiga, A.; Tanaka, T.; Orita, S.; Sato, A.; Sato, S.; Hachisu, T.; Abe, K.; Kimura, Y.; Kondo, Y.; Fujiwara, T.; et al.
Human T-cell leukemia virus type 1 protease protein expressed in Escherichia coli possesses aspartic proteinase activity (1993), Arch. Virol., 128, 195-210.

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Cloned(Commentary)

Cloned (Comment)	Organism
amplification of a HTLV-1 protease gene fragment by polymerase chain reaction and cloning it into a pUC plasmid vector. The protease gene fragment contains an open reading frame capable of encoding the active HTLV-1 protease. To express a fusion protein of beta-galactosidase linked with the HTLV-1 protease in Escherichia coli, a plasmid DNA is constructed by insering the HTLV-1 protease gen DNA into a procaryotic expression vector, pUEX-2, consisting of a lacZ gene directed by a lambda phage Pr promoter and designated pUEX-pro. The fusion protein is successfully expressed	Human T-cell leukemia virus type I

Cloned (Comment)

Organism

amplification of a HTLV-1 protease gene fragment by polymerase chain reaction and cloning it into a pUC plasmid vector. The protease gene fragment contains an open reading frame capable of encoding the active HTLV-1 protease. To express a fusion protein of beta-galactosidase linked with the HTLV-1 protease in Escherichia coli, a plasmid DNA is constructed by insering the HTLV-1 protease gen DNA into a procaryotic expression vector, pUEX-2, consisting of a lacZ gene directed by a lambda phage Pr promoter and designated pUEX-pro. The fusion protein is successfully expressed

Human T-cell leukemia virus type I

Inhibitors

Inhibitors	Comment	Organism	Structure
pepstatin A	-	Human T-cell leukemia virus type I

Organism

Organism	UniProt	Comment	Textmining
Human T-cell leukemia virus type I	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
gag precursor + H2O	HRTV-1 gag precursor polyprotein	Human T-cell leukemia virus type I	?	-	?

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Release 2023.1 (January 2023)