Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Mason-Pfizer monkey virus |
Protein Variants | Comment | Organism |
---|---|---|
A114R | site-directed mutagenesis, the mutation alters the substrate specificity and reduces the enzyme activity and viral infectivity of COS-1 cells | Mason-Pfizer monkey virus |
N109I/Q115I | site-directed mutagenesis, the mutation alters the substrate specificity and reduces the enzyme activity and viral infectivity of COS-1 cells | Mason-Pfizer monkey virus |
Q115I | site-directed mutagenesis, the mutation alters the substrate specificity and reduces the enzyme activity and viral infectivity of COS-1 cells | Mason-Pfizer monkey virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | activates at 0.3 M | Mason-Pfizer monkey virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mason-Pfizer monkey virus | - |
M-PMV | - |
Synonyms | Comment | Organism |
---|---|---|
M-PMV PR | - |
Mason-Pfizer monkey virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mason-Pfizer monkey virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
assay at | Mason-Pfizer monkey virus |