Literature summary for 3.4.23.B5 extracted from

Feher, A.; Boross, P.; Sperka, T.; Oroszlan, S.; Toezser, J.
Expression of the murine leukemia virus protease in fusion with maltose-binding protein in Escherichia coli (2004), Protein Expr. Purif., 35, 62-68.

Search for more literature for 3.4.23.B5

Cloned(Commentary)

Cloned (Comment)	Organism
expression as His-tagged maltose-binding protein fusion protein in Escherichia coli strains DH5alpha, CAG597, and BL21, optimization of the expression method to diminish protein degradation, overview, expression of His-tagged mutant D32A	murine leukemia virus

Protein Variants

Protein Variants	Comment	Organism
D32A	construction of an active site mutant which is inactive and more stable to degradation during recombinant expression and purification	murine leukemia virus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14100	-	purified recombinant enzyme, MALDI-TOF mass spectrometry	murine leukemia virus

Organism

Organism	UniProt	Comment	Textmining
murine leukemia virus	-	MLV	-
murine leukemia virus MLV	-	MLV	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant maltose-binding protein fusion enzyme from Escherichia coli strains DH5alpha and BL21, with or without His-tag, by amylose or nickel affinity chromatography, the fusion protein is cleaved of by factor Xa followed by cation exchange chromatography, non-His-tagged MBP-fusion-enzyme 2.6fold and His-tagged MBP-fusion enzyme 16.6fold to homogeneity, optimization of the purification method to diminish protein degradation, overview	murine leukemia virus

Purification (Comment)

Organism

recombinant maltose-binding protein fusion enzyme from Escherichia coli strains DH5alpha and BL21, with or without His-tag, by amylose or nickel affinity chromatography, the fusion protein is cleaved of by factor Xa followed by cation exchange chromatography, non-His-tagged MBP-fusion-enzyme 2.6fold and His-tagged MBP-fusion enzyme 16.6fold to homogeneity, optimization of the purification method to diminish protein degradation, overview

murine leukemia virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00054	-	purified recombinant non-tagged enzyme	murine leukemia virus
0.00112	-	purified recombinant His-tagged enzyme	murine leukemia virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme is active with the peptide representing the HIV-1 reverse transcriptase/integrase cleavage site, kinetics	murine leukemia virus	?	-	?
additional information	the enzyme is active with the peptide representing the HIV-1 reverse transcriptase/integrase cleavage site, kinetics	murine leukemia virus MLV	?	-	?

Synonyms

Synonyms	Comment	Organism
murine leukemia virus protease	-	murine leukemia virus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	murine leukemia virus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.6	-	assay at	murine leukemia virus